Duchardt-Ferner, Elke ; Gottstein-Schmidtke, Sina R. ; Weigand, Julia E. ; Ohlenschläger, Oliver ; Wurm, Jan-Philip ; Hammann, Christian ; Suess, Beatrix ; Wöhnert, Jens (2016)
What a Difference an OH Makes: Conformational Dynamics as the Basis for the Ligand Specificity of the Neomycin-Sensing Riboswitch.
In: Angewandte Chemie (International ed. in English), 55 (4)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
To ensure appropriate metabolic regulation, riboswitches must discriminate efficiently between their target ligands and chemically similar molecules that are also present in the cell. A remarkable example of efficient ligand discrimination is a synthetic neomycin-sensing riboswitch. Paromomycin, which differs from neomycin only by the substitution of a single amino group with a hydroxy group, also binds but does not flip the riboswitch. Interestingly, the solution structures of the two riboswitch-ligand complexes are virtually identical. In this work, we demonstrate that the local loss of key intermolecular interactions at the substitution site is translated through a defined network of intramolecular interactions into global changes in RNA conformational dynamics. The remarkable specificity of this riboswitch is thus based on structural dynamics rather than static structural differences. In this respect, the neomycin riboswitch is a model for many of its natural counterparts.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2016 |
| Autor(en): | Duchardt-Ferner, Elke ; Gottstein-Schmidtke, Sina R. ; Weigand, Julia E. ; Ohlenschläger, Oliver ; Wurm, Jan-Philip ; Hammann, Christian ; Suess, Beatrix ; Wöhnert, Jens |
| Art des Eintrags: | Bibliographie |
| Titel: | What a Difference an OH Makes: Conformational Dynamics as the Basis for the Ligand Specificity of the Neomycin-Sensing Riboswitch. |
| Sprache: | Englisch |
| Publikationsjahr: | 2016 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Angewandte Chemie (International ed. in English) |
| Jahrgang/Volume einer Zeitschrift: | 55 |
| (Heft-)Nummer: | 4 |
| Kurzbeschreibung (Abstract): | To ensure appropriate metabolic regulation, riboswitches must discriminate efficiently between their target ligands and chemically similar molecules that are also present in the cell. A remarkable example of efficient ligand discrimination is a synthetic neomycin-sensing riboswitch. Paromomycin, which differs from neomycin only by the substitution of a single amino group with a hydroxy group, also binds but does not flip the riboswitch. Interestingly, the solution structures of the two riboswitch-ligand complexes are virtually identical. In this work, we demonstrate that the local loss of key intermolecular interactions at the substitution site is translated through a defined network of intramolecular interactions into global changes in RNA conformational dynamics. The remarkable specificity of this riboswitch is thus based on structural dynamics rather than static structural differences. In this respect, the neomycin riboswitch is a model for many of its natural counterparts. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology") 10 Fachbereich Biologie > RNA Biochemie |
| Hinterlegungsdatum: | 22 Dez 2015 11:12 |
| Letzte Änderung: | 06 Nov 2020 07:11 |
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