Voss, Agnieszka ; Dietz, Christian ; Stocker, Annika ; Stark, Robert W. (2015):
Quantitative measurement of the mechanical properties of human antibodies with sub-10-nm resolution in a liquid environment.
In: Nano Research, 8 (6), pp. 1987-1996. TSINGHUA UNIV PRESS, TSINGHUA UNIV, BEIJING, PEOPLES R CHINA, ISSN 1998-0124,
[Article]
Abstract
The nanomechanical properties of single human immunoglobulin G and M antibodies were measured in a liquid environment using a fast force-volume technique with sub-10-nm spatial resolution. The ultrastructural details of these molecules were resolved in the images. Simultaneously, important physical properties, including elasticity, adhesion, and deformation were measured. The dimensions and adsorption of the immunoglobulin M antibodies onto the substrate indicated that they are highly flexible. The antibodies were characterized by a low elastic stiffness (34 +/- 10 MPa) and high deformability (1.5 +/- 0.5 nm).
| Item Type: | Article |
|---|---|
| Erschienen: | 2015 |
| Creators: | Voss, Agnieszka ; Dietz, Christian ; Stocker, Annika ; Stark, Robert W. |
| Title: | Quantitative measurement of the mechanical properties of human antibodies with sub-10-nm resolution in a liquid environment |
| Language: | English |
| Abstract: | The nanomechanical properties of single human immunoglobulin G and M antibodies were measured in a liquid environment using a fast force-volume technique with sub-10-nm spatial resolution. The ultrastructural details of these molecules were resolved in the images. Simultaneously, important physical properties, including elasticity, adhesion, and deformation were measured. The dimensions and adsorption of the immunoglobulin M antibodies onto the substrate indicated that they are highly flexible. The antibodies were characterized by a low elastic stiffness (34 +/- 10 MPa) and high deformability (1.5 +/- 0.5 nm). |
| Journal or Publication Title: | Nano Research |
| Journal Volume: | 8 |
| Issue Number: | 6 |
| Publisher: | TSINGHUA UNIV PRESS, TSINGHUA UNIV, BEIJING, PEOPLES R CHINA |
| Uncontrolled Keywords: | atomic force microscopy, structure and physical properties, piconewton forces, flexibility, antibodies, immunoglobulin |
| Divisions: | 11 Department of Materials and Earth Sciences > Material Science > Physics of Surfaces Zentrale Einrichtungen 11 Department of Materials and Earth Sciences > Material Science 11 Department of Materials and Earth Sciences Exzellenzinitiative Exzellenzinitiative > Clusters of Excellence Profile Areas > Thermo-Fluids & Interfaces Profile Areas |
| Date Deposited: | 31 Aug 2015 14:22 |
| URL / URN: | http://dx.doi.org/10.1007/s12274-015-0710-5 |
| Funders: | The authors thank the Center of Smart Interface for financial support. |
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