Cosentino, Cristian ; Alberio, Laura ; Gazzarrini, Sabrina ; Aquila, Marco ; Romano, Edoardo ; Cermenati, Solei ; Zuccolini, Paolo ; Petersen, Jan ; Beltrame, Monica ; Etten, James L. van ; Christie, John M. ; Thiel, Gerhard ; Moroni, Anna (2015)
Optogenetics. Engineering of a light-gated potassium channel.
In: Science (New York, N.Y.), 348 (6235)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The present palette of opsin-based optogenetic tools lacks a light-gated potassium (K(+)) channel desirable for silencing of excitable cells. Here, we describe the construction of a blue-light-induced K(+) channel 1 (BLINK1) engineered by fusing the plant LOV2-Jα photosensory module to the small viral K(+) channel Kcv. BLINK1 exhibits biophysical features of Kcv, including K(+) selectivity and high single-channel conductance but reversibly photoactivates in blue light. Opening of BLINK1 channels hyperpolarizes the cell to the K(+) equilibrium potential. Ectopic expression of BLINK1 reversibly inhibits the escape response in light-exposed zebrafish larvae. BLINK1 therefore provides a single-component optogenetic tool that can establish prolonged, physiological hyperpolarization of cells at low light intensities.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2015 |
| Autor(en): | Cosentino, Cristian ; Alberio, Laura ; Gazzarrini, Sabrina ; Aquila, Marco ; Romano, Edoardo ; Cermenati, Solei ; Zuccolini, Paolo ; Petersen, Jan ; Beltrame, Monica ; Etten, James L. van ; Christie, John M. ; Thiel, Gerhard ; Moroni, Anna |
| Art des Eintrags: | Bibliographie |
| Titel: | Optogenetics. Engineering of a light-gated potassium channel. |
| Sprache: | Deutsch |
| Publikationsjahr: | 2015 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Science (New York, N.Y.) |
| Jahrgang/Volume einer Zeitschrift: | 348 |
| (Heft-)Nummer: | 6235 |
| Kurzbeschreibung (Abstract): | The present palette of opsin-based optogenetic tools lacks a light-gated potassium (K(+)) channel desirable for silencing of excitable cells. Here, we describe the construction of a blue-light-induced K(+) channel 1 (BLINK1) engineered by fusing the plant LOV2-Jα photosensory module to the small viral K(+) channel Kcv. BLINK1 exhibits biophysical features of Kcv, including K(+) selectivity and high single-channel conductance but reversibly photoactivates in blue light. Opening of BLINK1 channels hyperpolarizes the cell to the K(+) equilibrium potential. Ectopic expression of BLINK1 reversibly inhibits the escape response in light-exposed zebrafish larvae. BLINK1 therefore provides a single-component optogenetic tool that can establish prolonged, physiological hyperpolarization of cells at low light intensities. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
| Hinterlegungsdatum: | 29 Jun 2015 12:44 |
| Letzte Änderung: | 20 Aug 2021 09:43 |
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