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T-DNA insertion in aquaporin gene AtPIP1;2 generates transcription profiles reminiscent of a low CO2 response.

Boudichevskaia, Anastassia and Heckwolf, Marlies and Kaldenhoff, Ralf (2015):
T-DNA insertion in aquaporin gene AtPIP1;2 generates transcription profiles reminiscent of a low CO2 response.
In: Plant, cell & environment, pp. 2286-2298, 38, (11), ISSN 1365-3040, [Article]

Abstract

Results from CO2 diffusion studies and characterization of Arabidopsis thaliana aquaporin AtPIP1;2 T-DNA insertion lines support the idea that specific aquaporins facilitate the diffusion of CO2 through biological membranes. However, their function as CO2 diffusion facilitators in plant physiology is still a matter of debate. Assuming that a lack of AtPIP1;2 causes a characteristic transcriptional response, we compared data from a AtPIP1;2 T-DNA insertion line obtained by Illumina sequencing, Affymetrix chip analysis and quantitative real time PCR to the transcriptome of plants grown under drought stress or under low CO2 conditions. The plant reaction to the deficit of AtPIP1;2 was unlike drought stress responses but comparable to that of low CO2 conditions. In addition, we observed a phenotype characteristic to plants grown under low CO2 . The findings support the hypothesis that AtPIP1;2 function in plant physiology is not to facilitate water but CO2 diffusion.

Item Type: Article
Erschienen: 2015
Creators: Boudichevskaia, Anastassia and Heckwolf, Marlies and Kaldenhoff, Ralf
Title: T-DNA insertion in aquaporin gene AtPIP1;2 generates transcription profiles reminiscent of a low CO2 response.
Language: English
Abstract:

Results from CO2 diffusion studies and characterization of Arabidopsis thaliana aquaporin AtPIP1;2 T-DNA insertion lines support the idea that specific aquaporins facilitate the diffusion of CO2 through biological membranes. However, their function as CO2 diffusion facilitators in plant physiology is still a matter of debate. Assuming that a lack of AtPIP1;2 causes a characteristic transcriptional response, we compared data from a AtPIP1;2 T-DNA insertion line obtained by Illumina sequencing, Affymetrix chip analysis and quantitative real time PCR to the transcriptome of plants grown under drought stress or under low CO2 conditions. The plant reaction to the deficit of AtPIP1;2 was unlike drought stress responses but comparable to that of low CO2 conditions. In addition, we observed a phenotype characteristic to plants grown under low CO2 . The findings support the hypothesis that AtPIP1;2 function in plant physiology is not to facilitate water but CO2 diffusion.

Journal or Publication Title: Plant, cell & environment
Volume: 38
Number: 11
Divisions: 10 Department of Biology
10 Department of Biology > Applied Plant Sciences
Date Deposited: 15 Apr 2015 06:11
Identification Number: doi:10.1111/pce.12547
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