TU Darmstadt / ULB / TUbiblio

The sorting of a small potassium channel in mammalian cells can be shifted between mitochondria and plasma membrane.

Chappuis, Charlotte von and Meckel, Tobias and Moroni, Anna and Thiel, Gerhard (2015):
The sorting of a small potassium channel in mammalian cells can be shifted between mitochondria and plasma membrane.
In: Cell calcium, pp. 114-121, 58, (1), ISSN 1532-1991,
[Article]

Abstract

The two small and similar viral K(+) channels Kcv and Kesv are sorted in mammalian cells and yeast to different destinations. Analysis of the sorting pathways shows that Kcv is trafficking via the secretory pathway to the plasma membrane, while Kesv is inserted via the TIM/TOM complex to the inner membrane of mitochondria. Studies with Kesv mutants show that an N-terminal mitochondrial targeting sequence in this channel is neither necessary nor sufficient for sorting of Kesv the mitochondria. Instead the sorting of Kesv can be redirected from the mitochondria to the plasma membrane by an insertion of ≥2 amino acids in a position sensitive manner into the C-terminal transmembrane domain (TMD2) of this channel. The available data advocate the presence of a C-terminal sorting signal in TMD2 of Kesv channel, which is presumably not determined by the length of this domain.

Item Type: Article
Erschienen: 2015
Creators: Chappuis, Charlotte von and Meckel, Tobias and Moroni, Anna and Thiel, Gerhard
Title: The sorting of a small potassium channel in mammalian cells can be shifted between mitochondria and plasma membrane.
Language: English
Abstract:

The two small and similar viral K(+) channels Kcv and Kesv are sorted in mammalian cells and yeast to different destinations. Analysis of the sorting pathways shows that Kcv is trafficking via the secretory pathway to the plasma membrane, while Kesv is inserted via the TIM/TOM complex to the inner membrane of mitochondria. Studies with Kesv mutants show that an N-terminal mitochondrial targeting sequence in this channel is neither necessary nor sufficient for sorting of Kesv the mitochondria. Instead the sorting of Kesv can be redirected from the mitochondria to the plasma membrane by an insertion of ≥2 amino acids in a position sensitive manner into the C-terminal transmembrane domain (TMD2) of this channel. The available data advocate the presence of a C-terminal sorting signal in TMD2 of Kesv channel, which is presumably not determined by the length of this domain.

Journal or Publication Title: Cell calcium
Volume: 58
Number: 1
Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophysics
10 Department of Biology > Membrane Dynamics
Date Deposited: 11 Dec 2014 10:00
Export:
Suche nach Titel in: TUfind oder in Google

Optionen (nur für Redakteure)

View Item View Item