Gutsze, A. ; Bodurka, J. ; Robert, O. ; Buntkowsky, G. ; Limbach, H. H. (1995)
H-1-Nmr and Calorimetric Measurements on Rabbit Eye Lenses.
In: Zeitschrift Fur Naturforschung C-a Journal of Biosciences, 50 (5-6)
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
The dynamic properties of water molecules in the rabbit lens were studied by proton nuclear magnetic resonance line shape analysis, measurements of relaxation times as a function of temperature and calorimetric measurements. The experiments prove, as already suggested by other authors, that there are two types of water in the lens of rabbit eyes, namely bound unfreezable hydration water and bulk freezable water. Line shape analysis and relaxometry showed, that this two types of water exist in two different environments, which may be identified as the nucleus and the cortex of the lens. The line shape analysis showed furthermore that water molecules in the rabbit lens has a common spin lattice relaxation time (T-1), but two different transverse relaxation times (T-2A and T-2B). The tentative model of fast water exchange on the T-1 time scale and slow water exchange on the T-2 time scale, was used to explain experimental proton relaxation data of the rabbit lens. An estimation for this exchange rate k(ex) by comparing it to the relaxation times is given (T-1(-1) much less than k(ex) much less than T-1(-1)). It has also been shown by a calorimetric measurements that the lenses can be easily undercooled to temperatures well below the freezing point of water. The achievable maximum undercooling temperature of the lens is a function of the cooling rate K-C, therefore it has to be considered as an experimentally adjustable parameter which is not characteristic for the investigated sample. Thus it must be noted that any previous discussions about the specific value of the temperature of water crystallisation in biological systems need to be carefully reconsidered.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1995 |
| Autor(en): | Gutsze, A. ; Bodurka, J. ; Robert, O. ; Buntkowsky, G. ; Limbach, H. H. |
| Art des Eintrags: | Bibliographie |
| Titel: | H-1-Nmr and Calorimetric Measurements on Rabbit Eye Lenses |
| Sprache: | Englisch |
| Publikationsjahr: | 1995 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Zeitschrift Fur Naturforschung C-a Journal of Biosciences |
| Jahrgang/Volume einer Zeitschrift: | 50 |
| (Heft-)Nummer: | 5-6 |
| URL / URN: | http://apps.webofknowledge.com/full_record.do?product=WOS&se... |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | The dynamic properties of water molecules in the rabbit lens were studied by proton nuclear magnetic resonance line shape analysis, measurements of relaxation times as a function of temperature and calorimetric measurements. The experiments prove, as already suggested by other authors, that there are two types of water in the lens of rabbit eyes, namely bound unfreezable hydration water and bulk freezable water. Line shape analysis and relaxometry showed, that this two types of water exist in two different environments, which may be identified as the nucleus and the cortex of the lens. The line shape analysis showed furthermore that water molecules in the rabbit lens has a common spin lattice relaxation time (T-1), but two different transverse relaxation times (T-2A and T-2B). The tentative model of fast water exchange on the T-1 time scale and slow water exchange on the T-2 time scale, was used to explain experimental proton relaxation data of the rabbit lens. An estimation for this exchange rate k(ex) by comparing it to the relaxation times is given (T-1(-1) much less than k(ex) much less than T-1(-1)). It has also been shown by a calorimetric measurements that the lenses can be easily undercooled to temperatures well below the freezing point of water. The achievable maximum undercooling temperature of the lens is a function of the cooling rate K-C, therefore it has to be considered as an experimentally adjustable parameter which is not characteristic for the investigated sample. Thus it must be noted that any previous discussions about the specific value of the temperature of water crystallisation in biological systems need to be carefully reconsidered. |
| Freie Schlagworte: | h-1 nmr rabbit lens relaxation measurements line shape water nuclear-magnetic-resonance protein solutions relaxation-times transverse relaxation cross-relaxation molecular theory tissue water temperature |
| Zusätzliche Informationen: | Rf993 Times Cited:8 Cited References Count:35 |
| Fachbereich(e)/-gebiet(e): | 07 Fachbereich Chemie 07 Fachbereich Chemie > Eduard Zintl-Institut 07 Fachbereich Chemie > Eduard Zintl-Institut > Fachgebiet Physikalische Chemie |
| Hinterlegungsdatum: | 27 Okt 2014 20:50 |
| Letzte Änderung: | 03 Jul 2024 02:22 |
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| Suche nach Titel in: | TUfind oder in Google |
Verfügbare Versionen dieses Eintrags
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¹H NMR and Calorimetric Measurements on Rabbit Eye Lenses. (deposited 07 Jan 2022 13:30)
- H-1-Nmr and Calorimetric Measurements on Rabbit Eye Lenses. (deposited 27 Okt 2014 20:50) [Gegenwärtig angezeigt]
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