Becker, Linda Carmen Annabelle (2014)
Influence of repair proteins and chromatin modifiers on mobility of DNA double-strand breaks induced by heavy ion irradiation.
Technische Universität Darmstadt
Dissertation, Erstveröffentlichung
Kurzbeschreibung (Abstract)
Ionizing radiation induces DNA double strand breaks (DSBs) in cell nuclei which represent the most dangerous type of DNA lesions. Through error prone repair mechanisms they can lead to the formation of chromosome rearrangements, favoring genomic instability and the development of cancer. Movement of DSBs and surrounding chromatin domains is expected to influence DSB repair and promote the formation of chromosomal translocations. Addressing this expectation, DSB mobility was analyzed after heavy ion irradiation. To gain insight into influences of chromatin organization and repair related factors on the dynamic behavior of DSB sites, knockdown or inhibition of repair proteins and chromatin modifying proteins was used. Occurring changes in irradiation induced foci (IRIF) mobility were examined either by immunofluorescent analysis of fixed samples or by live cell microscopy.
Typ des Eintrags: | Dissertation | ||||
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Erschienen: | 2014 | ||||
Autor(en): | Becker, Linda Carmen Annabelle | ||||
Art des Eintrags: | Erstveröffentlichung | ||||
Titel: | Influence of repair proteins and chromatin modifiers on mobility of DNA double-strand breaks induced by heavy ion irradiation | ||||
Sprache: | Englisch | ||||
Referenten: | Durante, Prof Marco ; Thiel, Prof Gerhard | ||||
Publikationsjahr: | 16 Mai 2014 | ||||
Ort: | Darmstadt | ||||
Datum der mündlichen Prüfung: | 16 Mai 2014 | ||||
URL / URN: | http://tuprints.ulb.tu-darmstadt.de/4038 | ||||
Kurzbeschreibung (Abstract): | Ionizing radiation induces DNA double strand breaks (DSBs) in cell nuclei which represent the most dangerous type of DNA lesions. Through error prone repair mechanisms they can lead to the formation of chromosome rearrangements, favoring genomic instability and the development of cancer. Movement of DSBs and surrounding chromatin domains is expected to influence DSB repair and promote the formation of chromosomal translocations. Addressing this expectation, DSB mobility was analyzed after heavy ion irradiation. To gain insight into influences of chromatin organization and repair related factors on the dynamic behavior of DSB sites, knockdown or inhibition of repair proteins and chromatin modifying proteins was used. Occurring changes in irradiation induced foci (IRIF) mobility were examined either by immunofluorescent analysis of fixed samples or by live cell microscopy. |
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URN: | urn:nbn:de:tuda-tuprints-40382 | ||||
Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie | ||||
Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Radiation Biology and DNA Repair |
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Hinterlegungsdatum: | 17 Aug 2014 19:55 | ||||
Letzte Änderung: | 17 Aug 2014 19:55 | ||||
PPN: | |||||
Referenten: | Durante, Prof Marco ; Thiel, Prof Gerhard | ||||
Datum der mündlichen Prüfung / Verteidigung / mdl. Prüfung: | 16 Mai 2014 | ||||
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