Braun, Christian J. ; Baer, Tom ; Moroni, Anna ; Thiel, Gerhard (2014)
Pseudo painting/air bubble technique for planar lipid bilayers.
In: Journal of neuroscience methods, 233
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
BACKGROUND
A functional reconstitution of channel proteins in planar lipid bilayers is still very versatile to study structure/function correlates under well-defined conditions at the single protein level.
NEW METHOD
In this study we present an improved planar lipid bilayer technique in which an air bubble is used for stabilizing unstable/leaky bilayers or for removing excess lipids. The bubble can also be used as a tool for reducing the number of channels in the bilayer with the goal of having only one active channel in the membrane.
RESULTS
Stable planar lipid bilayers are formed within seconds to minutes. In the case of multiple channel insertion the air bubble can be used to reduce the number of channels within minutes.
COMPARISON WITH EXISTING METHOD(S)
The simple improvement of the classical folding technique guarantees a very fast creation of stable bilayers even with difficult phospholipids in a conventional vertical bilayer set-up; it requires no modifications of the existing set-up.
CONCLUSIONS
This technique is very easy to handle and guarantees successful single channel recordings for any kind of planar lipid bilayer experiment.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2014 |
| Autor(en): | Braun, Christian J. ; Baer, Tom ; Moroni, Anna ; Thiel, Gerhard |
| Art des Eintrags: | Bibliographie |
| Titel: | Pseudo painting/air bubble technique for planar lipid bilayers. |
| Sprache: | Englisch |
| Publikationsjahr: | 2014 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of neuroscience methods |
| Jahrgang/Volume einer Zeitschrift: | 233 |
| Kurzbeschreibung (Abstract): | BACKGROUND A functional reconstitution of channel proteins in planar lipid bilayers is still very versatile to study structure/function correlates under well-defined conditions at the single protein level. NEW METHOD In this study we present an improved planar lipid bilayer technique in which an air bubble is used for stabilizing unstable/leaky bilayers or for removing excess lipids. The bubble can also be used as a tool for reducing the number of channels in the bilayer with the goal of having only one active channel in the membrane. RESULTS Stable planar lipid bilayers are formed within seconds to minutes. In the case of multiple channel insertion the air bubble can be used to reduce the number of channels within minutes. COMPARISON WITH EXISTING METHOD(S) The simple improvement of the classical folding technique guarantees a very fast creation of stable bilayers even with difficult phospholipids in a conventional vertical bilayer set-up; it requires no modifications of the existing set-up. CONCLUSIONS This technique is very easy to handle and guarantees successful single channel recordings for any kind of planar lipid bilayer experiment. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
| Hinterlegungsdatum: | 24 Jun 2014 11:48 |
| Letzte Änderung: | 14 Jul 2020 11:31 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
 |
Frage zum Eintrag |
Optionen (nur für Redakteure)
 |
Redaktionelle Details anzeigen |
Drucken
Impressum