Klein, Katharina ; Gigler, Alexander M. ; Aschenbrenner, Thomas ; Monetti, Roberto ; Bunk, Wolfram ; Jamitzky, Ferdinand ; Morfill, Gregor ; Stark, Robert W. ; Schlegel, Jürgen (2012)
Label-Free Live-Cell Imaging with Confocal Raman Microscopy.
In: Biophysical Journal, 102 (2)
doi: 10.1016/j.bpj.2011.12.027
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Confocal Raman spectroscopy is a noninvasive alternative to established cell imaging methods because it does not require chemical fixation, the use of fluorescent markers, or genetic engineering. In particular, single live-cell, high-resolution imaging by confocal Raman microscopy is desirable because it allows further experiments concerning the individually investigated cells. However, to derive meaningful images from the spectroscopic data, one must identify cell components within the dataset. Using immunofluorescence images as a reference, we derive Raman spectral signatures by means of information measures to identify cell components such as the nucleus, the endoplasmic reticulum, the Golgi apparatus, and mitochondria. The extracted signatures allow us to generate representations equivalent to conventional (immuno)fluorescence images with more than three cell components at a time, exploiting the Raman spectral information alone.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2012 |
| Autor(en): | Klein, Katharina ; Gigler, Alexander M. ; Aschenbrenner, Thomas ; Monetti, Roberto ; Bunk, Wolfram ; Jamitzky, Ferdinand ; Morfill, Gregor ; Stark, Robert W. ; Schlegel, Jürgen |
| Art des Eintrags: | Bibliographie |
| Titel: | Label-Free Live-Cell Imaging with Confocal Raman Microscopy |
| Sprache: | Englisch |
| Publikationsjahr: | 18 Januar 2012 |
| Verlag: | Elsevier Science Publishing |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biophysical Journal |
| Jahrgang/Volume einer Zeitschrift: | 102 |
| (Heft-)Nummer: | 2 |
| DOI: | 10.1016/j.bpj.2011.12.027 |
| Kurzbeschreibung (Abstract): | Confocal Raman spectroscopy is a noninvasive alternative to established cell imaging methods because it does not require chemical fixation, the use of fluorescent markers, or genetic engineering. In particular, single live-cell, high-resolution imaging by confocal Raman microscopy is desirable because it allows further experiments concerning the individually investigated cells. However, to derive meaningful images from the spectroscopic data, one must identify cell components within the dataset. Using immunofluorescence images as a reference, we derive Raman spectral signatures by means of information measures to identify cell components such as the nucleus, the endoplasmic reticulum, the Golgi apparatus, and mitochondria. The extracted signatures allow us to generate representations equivalent to conventional (immuno)fluorescence images with more than three cell components at a time, exploiting the Raman spectral information alone. |
| Fachbereich(e)/-gebiet(e): | 11 Fachbereich Material- und Geowissenschaften 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Physics of Surfaces DFG-Sonderforschungsbereiche (inkl. Transregio) DFG-Sonderforschungsbereiche (inkl. Transregio) > Sonderforschungsbereiche Exzellenzinitiative Exzellenzinitiative > Exzellenzcluster Zentrale Einrichtungen Exzellenzinitiative > Exzellenzcluster > Center of Smart Interfaces (CSI) |
| Hinterlegungsdatum: | 16 Jun 2014 09:31 |
| Letzte Änderung: | 21 Mär 2019 11:55 |
| PPN: | |
| Sponsoren: | This work was supported by the Deutsche Forschungsgemeinschaft Cluster of Excellence “Nanosystems Initiative Munich” and grant DFG-SFB-824 (Project B6: Imaging for Selection, Monitoring and Individualization of Cancer Therapies). |
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