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Blood platelet adhesion to printed von Willebrand factor

Davydovskaya, Polina and Janko, Marek and Gaertner, Florian and Ahmad, Zerkah and Simsek, Özlem and Maßberg, Steffen and Stark, Robert W. (2012):
Blood platelet adhesion to printed von Willebrand factor.
In: Journal of Biomedical Materials Research Part A, Wiley-VCH Verlag GmbH & Co. KGaA, pp. 335-341, 100A, (2), ISSN 15493296,
[Online-Edition: http://dx.doi.org/10.1002/jbm.a.33275],
[Article]

Abstract

Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma-treated glass and mica surfaces, producing elongated network-like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro.

Item Type: Article
Erschienen: 2012
Creators: Davydovskaya, Polina and Janko, Marek and Gaertner, Florian and Ahmad, Zerkah and Simsek, Özlem and Maßberg, Steffen and Stark, Robert W.
Title: Blood platelet adhesion to printed von Willebrand factor
Language: English
Abstract:

Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma-treated glass and mica surfaces, producing elongated network-like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro.

Journal or Publication Title: Journal of Biomedical Materials Research Part A
Volume: 100A
Number: 2
Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
Uncontrolled Keywords: von Willebrand factor, atomic force microscope, micro contact printing, blood platelet adhesion, lab on a chip
Divisions: 11 Department of Materials and Earth Sciences
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences > Material Science > Physics of Surfaces
Exzellenzinitiative
Exzellenzinitiative > Clusters of Excellence
Zentrale Einrichtungen
Exzellenzinitiative > Clusters of Excellence > Center of Smart Interfaces (CSI)
Date Deposited: 16 Jun 2014 09:21
Official URL: http://dx.doi.org/10.1002/jbm.a.33275
Identification Number: doi:10.1002/jbm.a.33275
Funders: Funded by Clusters of Excellence Center of Smart Interfaces Darmstadt and Nanosystems Initiative Munich, NIM
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