Davydovskaya, Polina ; Janko, Marek ; Gaertner, Florian ; Ahmad, Zerkah ; Simsek, Özlem ; Maßberg, Steffen ; Stark, Robert W. (2012)
Blood platelet adhesion to printed von Willebrand factor.
In: Journal of Biomedical Materials Research Part A, 100 (2)
doi: 10.1002/jbm.a.33275
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma-treated glass and mica surfaces, producing elongated network-like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2012 |
| Autor(en): | Davydovskaya, Polina ; Janko, Marek ; Gaertner, Florian ; Ahmad, Zerkah ; Simsek, Özlem ; Maßberg, Steffen ; Stark, Robert W. |
| Art des Eintrags: | Bibliographie |
| Titel: | Blood platelet adhesion to printed von Willebrand factor |
| Sprache: | Englisch |
| Publikationsjahr: | Februar 2012 |
| Verlag: | Wiley-VCH Verlag GmbH & Co. KGaA |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of Biomedical Materials Research Part A |
| Jahrgang/Volume einer Zeitschrift: | 100 |
| (Heft-)Nummer: | 2 |
| DOI: | 10.1002/jbm.a.33275 |
| Kurzbeschreibung (Abstract): | Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma-treated glass and mica surfaces, producing elongated network-like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro. |
| Freie Schlagworte: | von Willebrand factor, atomic force microscope, micro contact printing, blood platelet adhesion, lab on a chip |
| Fachbereich(e)/-gebiet(e): | 11 Fachbereich Material- und Geowissenschaften 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Physics of Surfaces Exzellenzinitiative Exzellenzinitiative > Exzellenzcluster Zentrale Einrichtungen Exzellenzinitiative > Exzellenzcluster > Center of Smart Interfaces (CSI) |
| Hinterlegungsdatum: | 16 Jun 2014 09:21 |
| Letzte Änderung: | 14 Jul 2020 11:27 |
| PPN: | |
| Sponsoren: | Funded by Clusters of Excellence Center of Smart Interfaces Darmstadt and Nanosystems Initiative Munich, NIM |
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