Davydovskaya, Polina ; Janko, Marek ; Gaertner, Florian ; Ahmad, Zerkah ; Simsek, Özlem ; Maßberg, Steffen ; Stark, Robert W. (2012):
Blood platelet adhesion to printed von Willebrand factor.
In: Journal of Biomedical Materials Research Part A, 100 (2), pp. 335-341. Wiley-VCH Verlag GmbH & Co. KGaA, ISSN 1549-3296,
[Article]
Abstract
Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma-treated glass and mica surfaces, producing elongated network-like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro.
| Item Type: | Article |
|---|---|
| Erschienen: | 2012 |
| Creators: | Davydovskaya, Polina ; Janko, Marek ; Gaertner, Florian ; Ahmad, Zerkah ; Simsek, Özlem ; Maßberg, Steffen ; Stark, Robert W. |
| Title: | Blood platelet adhesion to printed von Willebrand factor |
| Language: | English |
| Abstract: | Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma-treated glass and mica surfaces, producing elongated network-like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro. |
| Journal or Publication Title: | Journal of Biomedical Materials Research Part A |
| Journal volume: | 100 |
| Number: | 2 |
| Publisher: | Wiley-VCH Verlag GmbH & Co. KGaA |
| Uncontrolled Keywords: | von Willebrand factor, atomic force microscope, micro contact printing, blood platelet adhesion, lab on a chip |
| Divisions: | 11 Department of Materials and Earth Sciences 11 Department of Materials and Earth Sciences > Material Science 11 Department of Materials and Earth Sciences > Material Science > Physics of Surfaces Exzellenzinitiative Exzellenzinitiative > Clusters of Excellence Zentrale Einrichtungen Exzellenzinitiative > Clusters of Excellence > Center of Smart Interfaces (CSI) |
| Date Deposited: | 16 Jun 2014 09:21 |
| Official URL: | http://dx.doi.org/10.1002/jbm.a.33275 |
| Identification Number: | doi:10.1002/jbm.a.33275 |
| Funders: | Funded by Clusters of Excellence Center of Smart Interfaces Darmstadt and Nanosystems Initiative Munich, NIM |
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