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Production and consumption of nitrous oxide in nitrate-ammonifying Wolinella succinogenes cells.

Luckmann, Monique and Mania, Daniel and Kern, Melanie and Bakken, Lars R. and Frostegård, Asa and Simon, Jörg (2014):
Production and consumption of nitrous oxide in nitrate-ammonifying Wolinella succinogenes cells.
In: Microbiology (Reading, England), (160), pp. 1749-1759, ISSN 1465-2080,
[Article]

Abstract

Global warming is moving more and more to the public consciousness. Besides the commonly mentioned carbon dioxide and methane, nitrous oxide (N2O) is a powerful greenhouse gas in addition to its contribution to depletion of stratospheric ozone. The increasing concern about N2O emission has focused interest on underlying microbial energy converting processes and organisms harbouring N2O reductase (NosZ) such as denitrifiers and ammonifiers of nitrate and nitrite. Here, the epsilonproteobacterial model organism Wolinella succinogenes is investigated towards its capacity to produce and consume N2O during growth by anaerobic nitrate ammonification. This organism synthesizes an unconventional cytochrome c nitrous oxide reductase (cNosZ), which is encoded by the first gene of an atypical nos gene cluster. However, W. succinogenes lacks a nitric oxide (NO)-producing nitrite reductase of the NirS- or NirK-type as well as an NO reductase of the Nor-type. Using a robotized incubation system, the wild-type strain and suitable mutants of W. succinogenes that either produced or lacked cNosZ were analysed as to their production of NO, N2O and N2 in both nitrate-sufficient and nitrate-limited growth medium using formate as electron donor. It was found that cells growing in nitrate-sufficient medium produced small amounts of N2O that derived from nitrite and, most likely, from the presence of NO. Furthermore, cells employing cNosZ were able to reduce N2O to N2. This reaction, which was fully inhibited by acetylene, was also observed after adding N2O to the culture headspace. The results indicate that W. succinogenes cells are competent in N2O and N2 production despite being correctly grouped as respiratory nitrate ammonifiers. N2O production is assumed to result from NO detoxification and nitrosative stress defence while N2O serves as a terminal electron acceptor in anaerobic respiration. The ecological implications of these findings are discussed.

Item Type: Article
Erschienen: 2014
Creators: Luckmann, Monique and Mania, Daniel and Kern, Melanie and Bakken, Lars R. and Frostegård, Asa and Simon, Jörg
Title: Production and consumption of nitrous oxide in nitrate-ammonifying Wolinella succinogenes cells.
Language: English
Abstract:

Global warming is moving more and more to the public consciousness. Besides the commonly mentioned carbon dioxide and methane, nitrous oxide (N2O) is a powerful greenhouse gas in addition to its contribution to depletion of stratospheric ozone. The increasing concern about N2O emission has focused interest on underlying microbial energy converting processes and organisms harbouring N2O reductase (NosZ) such as denitrifiers and ammonifiers of nitrate and nitrite. Here, the epsilonproteobacterial model organism Wolinella succinogenes is investigated towards its capacity to produce and consume N2O during growth by anaerobic nitrate ammonification. This organism synthesizes an unconventional cytochrome c nitrous oxide reductase (cNosZ), which is encoded by the first gene of an atypical nos gene cluster. However, W. succinogenes lacks a nitric oxide (NO)-producing nitrite reductase of the NirS- or NirK-type as well as an NO reductase of the Nor-type. Using a robotized incubation system, the wild-type strain and suitable mutants of W. succinogenes that either produced or lacked cNosZ were analysed as to their production of NO, N2O and N2 in both nitrate-sufficient and nitrate-limited growth medium using formate as electron donor. It was found that cells growing in nitrate-sufficient medium produced small amounts of N2O that derived from nitrite and, most likely, from the presence of NO. Furthermore, cells employing cNosZ were able to reduce N2O to N2. This reaction, which was fully inhibited by acetylene, was also observed after adding N2O to the culture headspace. The results indicate that W. succinogenes cells are competent in N2O and N2 production despite being correctly grouped as respiratory nitrate ammonifiers. N2O production is assumed to result from NO detoxification and nitrosative stress defence while N2O serves as a terminal electron acceptor in anaerobic respiration. The ecological implications of these findings are discussed.

Journal or Publication Title: Microbiology (Reading, England)
Number: 160
Divisions: 10 Department of Biology
10 Department of Biology > Microbial Energy Conversion and Biotechnology
Date Deposited: 06 May 2014 09:01
Identification Number: doi:10.1099/mic.0.079293-0
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