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Metal Ion Affinity-based Biomolecular Recognition and Conjugation inside Synthetic Polymer Nanopores Modified with Iron–Terpyridine Complexes

Ali, Mubarak and Nasir, Saima and Nguyen, Quoc Hung and Sahoo, Jugal Kishore and Tahir, Muhammad Nawaz and Tremel, Wolfgang and Ensinger, Wolfgang (2011):
Metal Ion Affinity-based Biomolecular Recognition and Conjugation inside Synthetic Polymer Nanopores Modified with Iron–Terpyridine Complexes.
133, In: Journal of the American Chemical Society, (43), ACS Publications, pp. 17307-17314, ISSN 0002-7863, [Online-Edition: http://dx.doi.org/10.1021/ja205042t],
[Article]

Abstract

Here we demonstrate a novel biosensing platform for the detection of lactoferrin (LFN) via metal–organic frameworks, in which the metal ions have accessible free coordination sites for binding, inside the single conical nanopores fabricated in polymeric membrane. First, monolayer of amine-terminated terpyridine (metal–chelating ligand) is covalently immobilized on the inner walls of the nanopore via carbodiimide coupling chemistry. Second, iron–terpyridine (iron–terPy) complexes are obtained by treating the terpyridine modified-nanopores with ferrous sulfate solution. The immobilized iron–terPy complexes can be used as recognition elements to fabricate biosensing nanodevice. The working principle of the proposed biosensor is based on specific noncovalent interactions between LFN and chelated metal ions in the immobilized terpyridine monolayer, leading to the selective detection of analyte protein. In addition, control experiments proved that the designed biosensor exhibits excellent biospecificity and nonfouling properties. Furthermore, complementary experiments are conducted with multipore membranes containing an array of cylindrical nanopores. We demonstrate that in the presence of LFN in the feed solution, permeation of methyl viologen (MV2+) and 1,5-naphthalenedisulphate (NDS2–) is drastically suppressed across the iron–terPy modified membranes. On the basis of these findings, we envision that apart from conventional ligand–receptor interactions, the designing and immobilization of alternative functional ligands inside the synthetic nanopores would extend this method for the construction of new metal ion affinity-based biomimetic systems for the specific binding and recognition of other biomolecules.

Item Type: Article
Erschienen: 2011
Creators: Ali, Mubarak and Nasir, Saima and Nguyen, Quoc Hung and Sahoo, Jugal Kishore and Tahir, Muhammad Nawaz and Tremel, Wolfgang and Ensinger, Wolfgang
Title: Metal Ion Affinity-based Biomolecular Recognition and Conjugation inside Synthetic Polymer Nanopores Modified with Iron–Terpyridine Complexes
Language: English
Abstract:

Here we demonstrate a novel biosensing platform for the detection of lactoferrin (LFN) via metal–organic frameworks, in which the metal ions have accessible free coordination sites for binding, inside the single conical nanopores fabricated in polymeric membrane. First, monolayer of amine-terminated terpyridine (metal–chelating ligand) is covalently immobilized on the inner walls of the nanopore via carbodiimide coupling chemistry. Second, iron–terpyridine (iron–terPy) complexes are obtained by treating the terpyridine modified-nanopores with ferrous sulfate solution. The immobilized iron–terPy complexes can be used as recognition elements to fabricate biosensing nanodevice. The working principle of the proposed biosensor is based on specific noncovalent interactions between LFN and chelated metal ions in the immobilized terpyridine monolayer, leading to the selective detection of analyte protein. In addition, control experiments proved that the designed biosensor exhibits excellent biospecificity and nonfouling properties. Furthermore, complementary experiments are conducted with multipore membranes containing an array of cylindrical nanopores. We demonstrate that in the presence of LFN in the feed solution, permeation of methyl viologen (MV2+) and 1,5-naphthalenedisulphate (NDS2–) is drastically suppressed across the iron–terPy modified membranes. On the basis of these findings, we envision that apart from conventional ligand–receptor interactions, the designing and immobilization of alternative functional ligands inside the synthetic nanopores would extend this method for the construction of new metal ion affinity-based biomimetic systems for the specific binding and recognition of other biomolecules.

Journal or Publication Title: Journal of the American Chemical Society
Volume: 133
Number: 43
Publisher: ACS Publications
Divisions: 11 Department of Materials and Earth Sciences > Material Science > Material Analytics
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences
Date Deposited: 13 Dec 2012 08:07
Official URL: http://dx.doi.org/10.1021/ja205042t
Identification Number: doi:10.1021/ja205042t
Funders: M.A., S.N., Q.H.N., and W.E. gratefully acknowledge financial support by the Beilstein-Institut, Frankfurt/Main, Germany, within the research collaboration NanoBiC., M.N.T. and W.T. are thankful to the Deutsche Forschungsgemeinschaft, the Bundesministerium für Bildung und Forschung, Germany [Center of Excellence BIOTECmarin], the European Society for Marine Biotechnology for their financial support.
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