Smeazzetto, Serena ; Schröder, Indra ; Thiel, Gerhard ; Moncelli, Maria Rosa (2011)
Phospholamban generates cation selective ion channels.
In: Physical chemistry chemical physics : PCCP, 13 (28)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Phosholamban (PLN) is involved in the contractility of cardiac muscles by regulating the intracellular calcium concentration (Ca(2+)(cyt)) of cardiac myocytes. This occurs via a modulation of the sarco-/endoplasmic CaATPase (SERCA). In spite of high-resolution structures the molecular mode of PLN action is yet not known. In the present paper we readdress the question whether PLN proteins can generate ion channel activity. Reconstitution of PLN in planar lipid bilayers reveals single channel fluctuations, which are characterized by two conductance levels, long open/closed dwell times, moderate selectivity between monovalent cations and no perceivable Ca(2+) permeability. The PLN generated channel activity could be inhibited by a PLN antibody (abPLN) implying that the channel activity is indeed due to the inherent channel function of the PLN protein.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2011 |
| Autor(en): | Smeazzetto, Serena ; Schröder, Indra ; Thiel, Gerhard ; Moncelli, Maria Rosa |
| Art des Eintrags: | Bibliographie |
| Titel: | Phospholamban generates cation selective ion channels. |
| Sprache: | Englisch |
| Publikationsjahr: | 2011 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Physical chemistry chemical physics : PCCP |
| Jahrgang/Volume einer Zeitschrift: | 13 |
| (Heft-)Nummer: | 28 |
| Kurzbeschreibung (Abstract): | Phosholamban (PLN) is involved in the contractility of cardiac muscles by regulating the intracellular calcium concentration (Ca(2+)(cyt)) of cardiac myocytes. This occurs via a modulation of the sarco-/endoplasmic CaATPase (SERCA). In spite of high-resolution structures the molecular mode of PLN action is yet not known. In the present paper we readdress the question whether PLN proteins can generate ion channel activity. Reconstitution of PLN in planar lipid bilayers reveals single channel fluctuations, which are characterized by two conductance levels, long open/closed dwell times, moderate selectivity between monovalent cations and no perceivable Ca(2+) permeability. The PLN generated channel activity could be inhibited by a PLN antibody (abPLN) implying that the channel activity is indeed due to the inherent channel function of the PLN protein. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 25 Okt 2012 11:51 |
| Letzte Änderung: | 05 Mär 2013 10:03 |
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