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Uptake of fluorescent nano beads into BY2-cells involves clathrin-dependent and clathrin-independent endocytosis.

Bandmann, Vera ; Müller, Jasmin Daniela ; Köhler, Tim ; Homann, Ulrike (2012)
Uptake of fluorescent nano beads into BY2-cells involves clathrin-dependent and clathrin-independent endocytosis.
In: FEBS letters, 586 (20)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

To follow endocytosis in BY-2 cells we made use of fluorescent nano beads. Beads with 20nm in diameter were internalised rapidly and accumulated partially in compartments also labelled by the endocytic marker FM4-64. Studies in BY-2 cells and protoplasts revealed that larger beads (100nm) were excluded from uptake into turgescent and plasmolysed cells while protoplasts were able to internalise beads with a diameter of up to 1000nm. Endocytosis of beads was only partially inhibited by the clathrin-specific inhibitor Ikarugamycin and strongly blocked by wortmannin. These results imply that uptake of beads involves clathrin-dependent and clathrin-independent endocytic mechanisms and supports the hypothesis that clathrin-independent endocytosis plays a general role in plants.

Typ des Eintrags: Artikel
Erschienen: 2012
Autor(en): Bandmann, Vera ; Müller, Jasmin Daniela ; Köhler, Tim ; Homann, Ulrike
Art des Eintrags: Bibliographie
Titel: Uptake of fluorescent nano beads into BY2-cells involves clathrin-dependent and clathrin-independent endocytosis.
Sprache: Englisch
Publikationsjahr: 2012
Titel der Zeitschrift, Zeitung oder Schriftenreihe: FEBS letters
Jahrgang/Volume einer Zeitschrift: 586
(Heft-)Nummer: 20
Kurzbeschreibung (Abstract):

To follow endocytosis in BY-2 cells we made use of fluorescent nano beads. Beads with 20nm in diameter were internalised rapidly and accumulated partially in compartments also labelled by the endocytic marker FM4-64. Studies in BY-2 cells and protoplasts revealed that larger beads (100nm) were excluded from uptake into turgescent and plasmolysed cells while protoplasts were able to internalise beads with a diameter of up to 1000nm. Endocytosis of beads was only partially inhibited by the clathrin-specific inhibitor Ikarugamycin and strongly blocked by wortmannin. These results imply that uptake of beads involves clathrin-dependent and clathrin-independent endocytic mechanisms and supports the hypothesis that clathrin-independent endocytosis plays a general role in plants.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Pflanzliche Zellbiologie
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Hinterlegungsdatum: 16 Okt 2012 08:35
Letzte Änderung: 05 Mär 2013 10:03
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