Bandmann, Vera ; Müller, Jasmin Daniela ; Köhler, Tim ; Homann, Ulrike (2012):
Uptake of fluorescent nano beads into BY2-cells involves clathrin-dependent and clathrin-independent endocytosis.
In: FEBS letters, 586 (20), pp. 3626-3632. ISSN 1873-3468,
[Article]
Abstract
To follow endocytosis in BY-2 cells we made use of fluorescent nano beads. Beads with 20nm in diameter were internalised rapidly and accumulated partially in compartments also labelled by the endocytic marker FM4-64. Studies in BY-2 cells and protoplasts revealed that larger beads (100nm) were excluded from uptake into turgescent and plasmolysed cells while protoplasts were able to internalise beads with a diameter of up to 1000nm. Endocytosis of beads was only partially inhibited by the clathrin-specific inhibitor Ikarugamycin and strongly blocked by wortmannin. These results imply that uptake of beads involves clathrin-dependent and clathrin-independent endocytic mechanisms and supports the hypothesis that clathrin-independent endocytosis plays a general role in plants.
| Item Type: | Article |
|---|---|
| Erschienen: | 2012 |
| Creators: | Bandmann, Vera ; Müller, Jasmin Daniela ; Köhler, Tim ; Homann, Ulrike |
| Title: | Uptake of fluorescent nano beads into BY2-cells involves clathrin-dependent and clathrin-independent endocytosis. |
| Language: | English |
| Abstract: | To follow endocytosis in BY-2 cells we made use of fluorescent nano beads. Beads with 20nm in diameter were internalised rapidly and accumulated partially in compartments also labelled by the endocytic marker FM4-64. Studies in BY-2 cells and protoplasts revealed that larger beads (100nm) were excluded from uptake into turgescent and plasmolysed cells while protoplasts were able to internalise beads with a diameter of up to 1000nm. Endocytosis of beads was only partially inhibited by the clathrin-specific inhibitor Ikarugamycin and strongly blocked by wortmannin. These results imply that uptake of beads involves clathrin-dependent and clathrin-independent endocytic mechanisms and supports the hypothesis that clathrin-independent endocytosis plays a general role in plants. |
| Journal or Publication Title: | FEBS letters |
| Journal Volume: | 586 |
| Issue Number: | 20 |
| Divisions: | 10 Department of Biology 10 Department of Biology > Plant Cell Biology ?? fb10_botanik ?? |
| Date Deposited: | 16 Oct 2012 08:35 |
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