Zimmermann, U. ; Kaldenhoff, Ralf ; Hedrich, R. (2003)
Identifying feature-specific genes that in a donor plant provide a predetermined feature, useful for example for preparing transgenic plants colonizable by mycorrhiza, comprises screening fusions of donor and acceptor genetic materials.
Norm, Patent, Standard, Bibliographie

Kurzbeschreibung (Abstract)

Abstract: NOVELTY - Identifying feature-specific genes (A) that in a donor plant (D), provide a predetermined feature comprises:

(a) preparing genetic donor material from (D);

(b) preparing genetic receptor material from receptor plant (R) lacking the feature;

(c) heterologous fusion of the two to form hybrid material (H);

(d) culturing H and identifying model plants with the feature; and

(e) genetic analysis to identify those genes that are part of (D), as (A).

USE - The method is useful for identifying feature-specific genes (A) that in a donor plant (D), provide a predetermined feature (claimed). The method is used to identify feature-specific genes and to generate transgenic or chimeric plants with preselected features for use in forestry, agriculture, environmental protection, pharmaceuticals and production of active compounds. A particular feature is susceptibility to colonization by mycorrhizal fungi, which provide nutrients, trace elements and water, and increase resistance to e.g. heavy metals, but other features are e.g. ability to produce alginate; resistance to stressful conditions; or rapid growth, e.g. where genes from low-value trees are transferred to more valuable timber trees.

ADVANTAGE - The method is applicable to a large number of different features.

DETAILED DESCRIPTION - Identifying feature-specific genes (A) that, in a donor plant (D), cause appearance of a predetermined feature comprises:

(a) preparing genetic donor material from (D);

(b) preparing genetic receptor material from a receptor plant (R) that lacks the feature;

(c) heterologous fusion of the two materials to form hybrid material (H);

(d) culturing H and identifying at least one model plant with the feature; and

(e) genetic analysis of this plant to identify those genes that are part of (D), as (A).

INDEPENDENT CLAIMS are also included for the following:

(1) Genetic manipulation of plants in which a feature-specific gene, or gene cluster, is identified by the new method then transferred;

(2) Hybrid plants produced by method (1);

(3) Transgenic plants containing at least one (A) that has been identified by the new method; and

(4) Apparatus for identifying (A), especially by the new method.

Technology Focus/Extension Abstract: TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Process: Step (d) includes selection under conditions where hybrid material containing (A) has better survival than other hybrid material, especially culture in a physical or chemical medium against which only donor material is resistant. The donor material is genetically modified by incorporation of a known resistance gene into at least one chromosome (or segment) of (D). Selection may use promoter-reporter genes and the donor material has been subjected to chromosomal segregation, optionally also to chromosomal labeling by binding specific markers to chromosomes or their segments. Once an (A) has been detected, its location on particular chromosomes or segments of (D) is determined.Preferred Apparatus: This includes devices for preparing donor and receptor materials, fusion, and culture (including a selection step), and genetic analysis.

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