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Molecular convergence of the parasitic plant species Cuscuta reflexa and Phelipanche aegyptiaca.

Rehker, Jan and Lachnit, Magdalena and Kaldenhoff, Ralf (2012):
Molecular convergence of the parasitic plant species Cuscuta reflexa and Phelipanche aegyptiaca.
In: Planta, pp. 557-566, 236, (2), ISSN 1432-2048, [Article]

Abstract

The parasitic plant species Cuscuta reflexa and Phelipanche aegyptiaca have independently developed parasitism, the former parasitizing on shoots and the latter attaching to roots. Regardless of these differences, the two species use similar organs, termed haustoria, to attach to the host plant. In this study, we show that this morphological similarity can be extended to the molecular level. An attAGP-promoter from Solanum lycopersicum, which is activated by Cuscuta infections, was also induced after infection by P. aegyptiaca. Furthermore, we show by validation of transcriptome sequencing data that the Phelipanche orthologue of a haustorium-specific Cuscuta gene, which codes for a cysteine proteinase, was activated in the early stages of Phelipanche invasion. Inhibition of the Phelipanche cysteine proteinase was achieved by 35S- or attAGP-promoter-driven expression of its intrinsic inhibitory polypeptide. A reduction in P. aegyptiaca infection rates during experiments in flower pots and in an in vitro polybag system in comparison to controls was recorded.

Item Type: Article
Erschienen: 2012
Creators: Rehker, Jan and Lachnit, Magdalena and Kaldenhoff, Ralf
Title: Molecular convergence of the parasitic plant species Cuscuta reflexa and Phelipanche aegyptiaca.
Language: English
Abstract:

The parasitic plant species Cuscuta reflexa and Phelipanche aegyptiaca have independently developed parasitism, the former parasitizing on shoots and the latter attaching to roots. Regardless of these differences, the two species use similar organs, termed haustoria, to attach to the host plant. In this study, we show that this morphological similarity can be extended to the molecular level. An attAGP-promoter from Solanum lycopersicum, which is activated by Cuscuta infections, was also induced after infection by P. aegyptiaca. Furthermore, we show by validation of transcriptome sequencing data that the Phelipanche orthologue of a haustorium-specific Cuscuta gene, which codes for a cysteine proteinase, was activated in the early stages of Phelipanche invasion. Inhibition of the Phelipanche cysteine proteinase was achieved by 35S- or attAGP-promoter-driven expression of its intrinsic inhibitory polypeptide. A reduction in P. aegyptiaca infection rates during experiments in flower pots and in an in vitro polybag system in comparison to controls was recorded.

Journal or Publication Title: Planta
Volume: 236
Number: 2
Divisions: 10 Department of Biology > Applied Plant Sciences
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10 Department of Biology
Date Deposited: 05 Apr 2012 08:37
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