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Observing conformational and activity changes of tet repressor in vivo.

Tiebel, Beatrix and Garke, K. and Hillen, W. (2000):
Observing conformational and activity changes of tet repressor in vivo.
7, In: Nature structural biology, (6), pp. 479-81, ISSN 1072-8368, [Article]

Abstract

Effector triggered conformational changes of proteins such as regulators of transcription, receptors, or enzymes are the molecular basis for regulation in biology. Most proteins perform their biological functions intracellularly, in the presence of many potential interaction partners. Studies of conformational changes have mainly been performed in vitro using sophisticated physical and biochemical methods that usually require purified proteins. Here we describe the observation of conformational changes of Tet repressor in the cytoplasm of growing Escherichia coli cells, analyzed by ligand dependent disulfide crosslinking of cysteine residues substituted into mobile regions of the protein. The amount of protein undergoing the structural change is quantitatively linked to the concomitant induction of transcription of a reporter gene.

Item Type: Article
Erschienen: 2000
Creators: Tiebel, Beatrix and Garke, K. and Hillen, W.
Title: Observing conformational and activity changes of tet repressor in vivo.
Language: English
Abstract:

Effector triggered conformational changes of proteins such as regulators of transcription, receptors, or enzymes are the molecular basis for regulation in biology. Most proteins perform their biological functions intracellularly, in the presence of many potential interaction partners. Studies of conformational changes have mainly been performed in vitro using sophisticated physical and biochemical methods that usually require purified proteins. Here we describe the observation of conformational changes of Tet repressor in the cytoplasm of growing Escherichia coli cells, analyzed by ligand dependent disulfide crosslinking of cysteine residues substituted into mobile regions of the protein. The amount of protein undergoing the structural change is quantitatively linked to the concomitant induction of transcription of a reporter gene.

Journal or Publication Title: Nature structural biology
Volume: 7
Number: 6
Divisions: 10 Department of Biology > Synthetic Genetic Circuits
10 Department of Biology
Date Deposited: 22 Feb 2012 10:46
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