Krstić, Ivan ; Frolow, Olga ; Sezer, Deniz ; Endeward, Burkhard ; Weigand, Julia E. ; Suess, Beatrix ; Engels, Joachim W. ; Prisner, Thomas F. (2010)
PELDOR spectroscopy reveals preorganization of the neomycin-responsive riboswitch tertiary structure.
In: Journal of the American Chemical Society, 132 (5)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Pulsed electron double resonance (PELDOR) spectroscopy reveals a prearranged tertiary structure of the 27 nucleotides long engineered neomycin-responsive riboswitch. Measured distances between spin labels at positions U4-U14, U4-U15, U14-U26, and U15-U26 were unchanged upon neomycin binding which implies that the global stem-loop architecture is preserved in the absence and presence of the ligand. On the basis of our results, we infer that low-temperature PELDOR data unambiguously demonstrate the existence of an enthalpically favorable set of RNA conformations ready to bind the ligand without major global rearrangement.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2010 |
| Autor(en): | Krstić, Ivan ; Frolow, Olga ; Sezer, Deniz ; Endeward, Burkhard ; Weigand, Julia E. ; Suess, Beatrix ; Engels, Joachim W. ; Prisner, Thomas F. |
| Art des Eintrags: | Bibliographie |
| Titel: | PELDOR spectroscopy reveals preorganization of the neomycin-responsive riboswitch tertiary structure. |
| Sprache: | Englisch |
| Publikationsjahr: | 2010 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of the American Chemical Society |
| Jahrgang/Volume einer Zeitschrift: | 132 |
| (Heft-)Nummer: | 5 |
| Kurzbeschreibung (Abstract): | Pulsed electron double resonance (PELDOR) spectroscopy reveals a prearranged tertiary structure of the 27 nucleotides long engineered neomycin-responsive riboswitch. Measured distances between spin labels at positions U4-U14, U4-U15, U14-U26, and U15-U26 were unchanged upon neomycin binding which implies that the global stem-loop architecture is preserved in the absence and presence of the ligand. On the basis of our results, we infer that low-temperature PELDOR data unambiguously demonstrate the existence of an enthalpically favorable set of RNA conformations ready to bind the ligand without major global rearrangement. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology") 10 Fachbereich Biologie > RNA Biochemie |
| Hinterlegungsdatum: | 22 Feb 2012 10:25 |
| Letzte Änderung: | 06 Nov 2020 07:20 |
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