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Selection of tetracycline inducible self-cleaving ribozymes as synthetic devices for gene regulation in yeast.

Wittmann, Alexander and Suess, Beatrix (2011):
Selection of tetracycline inducible self-cleaving ribozymes as synthetic devices for gene regulation in yeast.
7, In: Molecular bioSystems, (8), pp. 2419-27, ISSN 1742-2051, [Article]

Abstract

Synthetic regulatory devices are key components for the development of complex biological systems and the reprogramming of cellular functions and networks. Here we describe the selection of tetracycline inducible hammerhead ribozymes. A tetracycline aptamer was fused to the full-length hammerhead ribozyme via a variable linker region. 11 rounds of in vitro selection were applied to isolate linker sequences that mediate tetracycline dependent hammerhead cleavage. We identified allosteric ribozymes that cleave in the presence of 1 μM tetracycline as fast as the full-length ribozyme whereas cleavage is inhibited up to 333-fold in the absence of tetracycline. Reporter gene assays indicate that the allosteric ribozymes can be employed to control gene expression in yeast.

Item Type: Article
Erschienen: 2011
Creators: Wittmann, Alexander and Suess, Beatrix
Title: Selection of tetracycline inducible self-cleaving ribozymes as synthetic devices for gene regulation in yeast.
Language: English
Abstract:

Synthetic regulatory devices are key components for the development of complex biological systems and the reprogramming of cellular functions and networks. Here we describe the selection of tetracycline inducible hammerhead ribozymes. A tetracycline aptamer was fused to the full-length hammerhead ribozyme via a variable linker region. 11 rounds of in vitro selection were applied to isolate linker sequences that mediate tetracycline dependent hammerhead cleavage. We identified allosteric ribozymes that cleave in the presence of 1 μM tetracycline as fast as the full-length ribozyme whereas cleavage is inhibited up to 333-fold in the absence of tetracycline. Reporter gene assays indicate that the allosteric ribozymes can be employed to control gene expression in yeast.

Journal or Publication Title: Molecular bioSystems
Volume: 7
Number: 8
Divisions: 10 Department of Biology > Synthetic Genetic Circuits
10 Department of Biology
Date Deposited: 22 Feb 2012 10:15
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