Wittmann, Alexander ; Suess, Beatrix (2011)
Selection of tetracycline inducible self-cleaving ribozymes as synthetic devices for gene regulation in yeast.
In: Molecular bioSystems, 7 (8)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Synthetic regulatory devices are key components for the development of complex biological systems and the reprogramming of cellular functions and networks. Here we describe the selection of tetracycline inducible hammerhead ribozymes. A tetracycline aptamer was fused to the full-length hammerhead ribozyme via a variable linker region. 11 rounds of in vitro selection were applied to isolate linker sequences that mediate tetracycline dependent hammerhead cleavage. We identified allosteric ribozymes that cleave in the presence of 1 μM tetracycline as fast as the full-length ribozyme whereas cleavage is inhibited up to 333-fold in the absence of tetracycline. Reporter gene assays indicate that the allosteric ribozymes can be employed to control gene expression in yeast.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2011 |
| Autor(en): | Wittmann, Alexander ; Suess, Beatrix |
| Art des Eintrags: | Bibliographie |
| Titel: | Selection of tetracycline inducible self-cleaving ribozymes as synthetic devices for gene regulation in yeast. |
| Sprache: | Englisch |
| Publikationsjahr: | 2011 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Molecular bioSystems |
| Jahrgang/Volume einer Zeitschrift: | 7 |
| (Heft-)Nummer: | 8 |
| Kurzbeschreibung (Abstract): | Synthetic regulatory devices are key components for the development of complex biological systems and the reprogramming of cellular functions and networks. Here we describe the selection of tetracycline inducible hammerhead ribozymes. A tetracycline aptamer was fused to the full-length hammerhead ribozyme via a variable linker region. 11 rounds of in vitro selection were applied to isolate linker sequences that mediate tetracycline dependent hammerhead cleavage. We identified allosteric ribozymes that cleave in the presence of 1 μM tetracycline as fast as the full-length ribozyme whereas cleavage is inhibited up to 333-fold in the absence of tetracycline. Reporter gene assays indicate that the allosteric ribozymes can be employed to control gene expression in yeast. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology") 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 22 Feb 2012 10:15 |
| Letzte Änderung: | 05 Mär 2013 09:58 |
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