Brand, Michael ; Sommer, Matthias ; Achenbach, Stephan ; Anders, Katharina ; Lell, Michael ; Löbrich, Markus ; Uder, Michael ; Kuefner, Michael A. (2012)
X-ray induced DNA double-strand breaks in coronary CT angiography: Comparison of sequential, low-pitch helical and high-pitch helical data acquisition.
In: European journal of radiology, 81 (3)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
BACKGROUND: Aim of this study was to compare DNA double-strand breaks (DSBs) in blood lymphocytes of patients undergoing high-pitch helical, low-pitch helical and sequential coronary CT angiography. METHODS AND RESULTS: 66 patients were examined with various scan protocols and modes (low-pitch helical scan: 100-120kV, 320-438mAs/rot, pitch 0.18-0.39, with or without ECG-pulsing, n=35; prospectively ECG-triggered high-pitch helical scan: 100-120kV, 320-456mAs/rotation, pitch 3.2-3.4, n=19; prospectively ECG-triggered sequential scan: 100-120kV, 150-300mAs or 320-370mAs/rotation, n=12) either using a 64-slice or 128-slice dual-source CT or a 128-slice single source CT scanner. Blood samples were obtained before and 30min after CT and DSBs were analyzed in isolated lymphocytes using γ-H2AX immunofluorescence microscopy. A significant increase of DSBs was measurable 30min after CTA (range 0.01-0.71/cell). CT induced DSBs showed a significant correlation with the estimated effective dose (ρ=0.90, p<0.00001). Both prospectively ECG-triggered sequential (0.10DSBs/cell, 176mGycm, p<0.00001) and high-pitch helical scan protocols (0.03DSBs/cell, 109mGycm, p<0.00001) led to a significant reduction of median DLP and DSB levels compared to low-pitch helical scans (0.34DSBs/cell, 828mGycm). A reduction of the tube voltage resulted in significantly lower whereas additional calcium scoring resulted in elevated DLP and DNA damages (p<0.05 each). CONCLUSION: In coronary CTA, data acquisition protocols have a significant influence on the X-ray induced DSB levels. Using γ-H2AX immunofluorescence microscopy different scan modes in different CT generations can be compared concerning their biological impact.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2012 |
| Autor(en): | Brand, Michael ; Sommer, Matthias ; Achenbach, Stephan ; Anders, Katharina ; Lell, Michael ; Löbrich, Markus ; Uder, Michael ; Kuefner, Michael A. |
| Art des Eintrags: | Bibliographie |
| Titel: | X-ray induced DNA double-strand breaks in coronary CT angiography: Comparison of sequential, low-pitch helical and high-pitch helical data acquisition. |
| Sprache: | Englisch |
| Publikationsjahr: | 2012 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | European journal of radiology |
| Jahrgang/Volume einer Zeitschrift: | 81 |
| (Heft-)Nummer: | 3 |
| Kurzbeschreibung (Abstract): | BACKGROUND: Aim of this study was to compare DNA double-strand breaks (DSBs) in blood lymphocytes of patients undergoing high-pitch helical, low-pitch helical and sequential coronary CT angiography. METHODS AND RESULTS: 66 patients were examined with various scan protocols and modes (low-pitch helical scan: 100-120kV, 320-438mAs/rot, pitch 0.18-0.39, with or without ECG-pulsing, n=35; prospectively ECG-triggered high-pitch helical scan: 100-120kV, 320-456mAs/rotation, pitch 3.2-3.4, n=19; prospectively ECG-triggered sequential scan: 100-120kV, 150-300mAs or 320-370mAs/rotation, n=12) either using a 64-slice or 128-slice dual-source CT or a 128-slice single source CT scanner. Blood samples were obtained before and 30min after CT and DSBs were analyzed in isolated lymphocytes using γ-H2AX immunofluorescence microscopy. A significant increase of DSBs was measurable 30min after CTA (range 0.01-0.71/cell). CT induced DSBs showed a significant correlation with the estimated effective dose (ρ=0.90, p<0.00001). Both prospectively ECG-triggered sequential (0.10DSBs/cell, 176mGycm, p<0.00001) and high-pitch helical scan protocols (0.03DSBs/cell, 109mGycm, p<0.00001) led to a significant reduction of median DLP and DSB levels compared to low-pitch helical scans (0.34DSBs/cell, 828mGycm). A reduction of the tube voltage resulted in significantly lower whereas additional calcium scoring resulted in elevated DLP and DNA damages (p<0.05 each). CONCLUSION: In coronary CTA, data acquisition protocols have a significant influence on the X-ray induced DSB levels. Using γ-H2AX immunofluorescence microscopy different scan modes in different CT generations can be compared concerning their biological impact. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Radiation Biology and DNA Repair |
| Hinterlegungsdatum: | 25 Jan 2012 07:29 |
| Letzte Änderung: | 01 Mär 2017 07:41 |
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