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Secondary structure is required for 3' splice site recognition in yeast.

Gahura, Ondrej and Hammann, Christian and Valentová, Anna and Puta, Frantisek and Folk, Petr (2011):
Secondary structure is required for 3' splice site recognition in yeast.
In: Nucleic acids research, pp. 9759-9767, 39, (22), ISSN 1362-4962, [Article]

Abstract

Higher order RNA structures can mask splicing signals, loop out exons, or constitute riboswitches all of which contributes to the complexity of splicing regulation. We identified a G to A substitution between branch point (BP) and 3' splice site (3'ss) of Saccharomyces cerevisiae COF1 intron, which dramatically impaired its splicing. RNA structure prediction and in-line probing showed that this mutation disrupted a stem in the BP-3'ss region. Analyses of various COF1 intron modifications revealed that the secondary structure brought about the reduction of BP to 3'ss distance and masked potential 3'ss. We demonstrated the same structural requisite for the splicing of UBC13 intron. Moreover, RNAfold predicted stable structures for almost all distant BP introns in S. cerevisiae and for selected examples in several other Saccharomycotina species. The employment of intramolecular structure to localize 3'ss for the second splicing step suggests the existence of pre-mRNA structure-based mechanism of 3'ss recognition.

Item Type: Article
Erschienen: 2011
Creators: Gahura, Ondrej and Hammann, Christian and Valentová, Anna and Puta, Frantisek and Folk, Petr
Title: Secondary structure is required for 3' splice site recognition in yeast.
Language: English
Abstract:

Higher order RNA structures can mask splicing signals, loop out exons, or constitute riboswitches all of which contributes to the complexity of splicing regulation. We identified a G to A substitution between branch point (BP) and 3' splice site (3'ss) of Saccharomyces cerevisiae COF1 intron, which dramatically impaired its splicing. RNA structure prediction and in-line probing showed that this mutation disrupted a stem in the BP-3'ss region. Analyses of various COF1 intron modifications revealed that the secondary structure brought about the reduction of BP to 3'ss distance and masked potential 3'ss. We demonstrated the same structural requisite for the splicing of UBC13 intron. Moreover, RNAfold predicted stable structures for almost all distant BP introns in S. cerevisiae and for selected examples in several other Saccharomycotina species. The employment of intramolecular structure to localize 3'ss for the second splicing step suggests the existence of pre-mRNA structure-based mechanism of 3'ss recognition.

Journal or Publication Title: Nucleic acids research
Volume: 39
Number: 22
Divisions: 10 Department of Biology > Ribogenetics
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10 Department of Biology
Date Deposited: 13 Sep 2011 06:17
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