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Ca(2)+-stimulated exocytosis in maize coleoptile cells.

Sutter, J. U. ; Homann, Ulrike ; Thiel, Gerhard (2000)
Ca(2)+-stimulated exocytosis in maize coleoptile cells.
In: The Plant cell, 12 (7)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Changes in membrane capacitance (C(m)) after photolysis of the caged Ca(2)+ compound dimethoxynitrophenamine were studied in protoplasts from maize coleoptiles. Changes in C(m) values resulting from increased concentrations of free Ca(2)+ in the cytoplasm ([Ca(2)+](cyt)) were interpreted as representing changes in [Ca(2)+](cyt)-sensitive exocytosis and endocytosis. A continuous increase in [Ca(2)+](cyt) resulted in a sigmoidal increase in C(m) values with a half-maximal concentration at approximately 1 microM. The steep increase in C(m) values was followed by a variable slow phase in changing C(m) values. When [Ca(2)+](cyt) increased at a rate of 0.6 micromol L(-)(1) sec(-)(1), the initial steep increase in C(m) values lasted approximately 5 to 10 sec. During this time, protoplasts increased in surface area by approximately 2.5%. The biphasic dynamics of [Ca(2)+](cyt)-stimulated increases in C(m) values can be described by a kinetic model containing two pools of vesicles with two [Ca(2)+](cyt)-sensitive steps in the exocytotic pathway.

Typ des Eintrags: Artikel
Erschienen: 2000
Autor(en): Sutter, J. U. ; Homann, Ulrike ; Thiel, Gerhard
Art des Eintrags: Bibliographie
Titel: Ca(2)+-stimulated exocytosis in maize coleoptile cells.
Sprache: Englisch
Publikationsjahr: 2000
Titel der Zeitschrift, Zeitung oder Schriftenreihe: The Plant cell
Jahrgang/Volume einer Zeitschrift: 12
(Heft-)Nummer: 7
Kurzbeschreibung (Abstract):

Changes in membrane capacitance (C(m)) after photolysis of the caged Ca(2)+ compound dimethoxynitrophenamine were studied in protoplasts from maize coleoptiles. Changes in C(m) values resulting from increased concentrations of free Ca(2)+ in the cytoplasm ([Ca(2)+](cyt)) were interpreted as representing changes in [Ca(2)+](cyt)-sensitive exocytosis and endocytosis. A continuous increase in [Ca(2)+](cyt) resulted in a sigmoidal increase in C(m) values with a half-maximal concentration at approximately 1 microM. The steep increase in C(m) values was followed by a variable slow phase in changing C(m) values. When [Ca(2)+](cyt) increased at a rate of 0.6 micromol L(-)(1) sec(-)(1), the initial steep increase in C(m) values lasted approximately 5 to 10 sec. During this time, protoplasts increased in surface area by approximately 2.5%. The biphasic dynamics of [Ca(2)+](cyt)-stimulated increases in C(m) values can be described by a kinetic model containing two pools of vesicles with two [Ca(2)+](cyt)-sensitive steps in the exocytotic pathway.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
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10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen)
10 Fachbereich Biologie > Pflanzliche Zellbiologie
Hinterlegungsdatum: 22 Jun 2011 09:23
Letzte Änderung: 05 Mär 2013 09:49
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