Homann, Ulrike ; Thiel, Gerhard (1999)
Unitary exocytotic and endocytotic events in guard-cell protoplasts during osmotically driven volume changes.
In: FEBS letters, 460 (3)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Osmotically driven swelling and shrinking of guard-cell protoplasts (GCPs) requires adjustment of surface area which is achieved by addition and removal of plasma membrane material. To investigate the mechanism for adaptation of surface area we have used patch-clamp capacitance measurements. The recorded membrane capacitance (C(m)) trace of swelling and shrinking GCPs occasionally revealed discrete upward and downward deflecting capacitance steps, respectively, with a median value of about 2 fF. The observed capacitance steps resulted from the fusion and fission of single vesicles with a diameter of around 300 nm. We conclude that exo- and endocytosis of these vesicles accommodate for osmotically driven surface area changes in GCPs.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1999 |
| Autor(en): | Homann, Ulrike ; Thiel, Gerhard |
| Art des Eintrags: | Bibliographie |
| Titel: | Unitary exocytotic and endocytotic events in guard-cell protoplasts during osmotically driven volume changes. |
| Sprache: | Englisch |
| Publikationsjahr: | 1999 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | FEBS letters |
| Jahrgang/Volume einer Zeitschrift: | 460 |
| (Heft-)Nummer: | 3 |
| Kurzbeschreibung (Abstract): | Osmotically driven swelling and shrinking of guard-cell protoplasts (GCPs) requires adjustment of surface area which is achieved by addition and removal of plasma membrane material. To investigate the mechanism for adaptation of surface area we have used patch-clamp capacitance measurements. The recorded membrane capacitance (C(m)) trace of swelling and shrinking GCPs occasionally revealed discrete upward and downward deflecting capacitance steps, respectively, with a median value of about 2 fF. The observed capacitance steps resulted from the fusion and fission of single vesicles with a diameter of around 300 nm. We conclude that exo- and endocytosis of these vesicles accommodate for osmotically driven surface area changes in GCPs. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_botanik ?? 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) 10 Fachbereich Biologie > Pflanzliche Zellbiologie |
| Hinterlegungsdatum: | 22 Jun 2011 07:42 |
| Letzte Änderung: | 05 Mär 2013 09:49 |
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