Thiel, Gerhard ; Sutter, J. U. ; Homann, Ulrike (2000)
Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleoptile protoplasts.
In: Pflügers Archiv : European journal of physiology, 439 (3 Supp)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Ca2+ and osmotic driven extension of the surface area of maize coleoptile protoplasts was investigated using capacitance measurements and photolysis of the caged compound DM-nitrophen. Protoplasts responded to an elevation of cytoplasmic Ca2+ (Ca(i)) with a rapid burst in capacitance reaching a maximal increase of 1.3+/-1.1% over the resting cell capacitance. Subsequent lowering of the osmotic potential in the external medium by 210 mosmol caused a further increase in Cm by 26+/-6%. These data indicate two independent pathways for insertion of membrane into the plasma membrane. One is driven by Ca(i) and recruits membrane from a small pool. The osmotic evoked rise in surface area draws membrane from a much larger reservoir and may be driven by membrane tension.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2000 |
| Autor(en): | Thiel, Gerhard ; Sutter, J. U. ; Homann, Ulrike |
| Art des Eintrags: | Bibliographie |
| Titel: | Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleoptile protoplasts. |
| Sprache: | Englisch |
| Publikationsjahr: | 2000 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Pflügers Archiv : European journal of physiology |
| Jahrgang/Volume einer Zeitschrift: | 439 |
| (Heft-)Nummer: | 3 Supp |
| Kurzbeschreibung (Abstract): | Ca2+ and osmotic driven extension of the surface area of maize coleoptile protoplasts was investigated using capacitance measurements and photolysis of the caged compound DM-nitrophen. Protoplasts responded to an elevation of cytoplasmic Ca2+ (Ca(i)) with a rapid burst in capacitance reaching a maximal increase of 1.3+/-1.1% over the resting cell capacitance. Subsequent lowering of the osmotic potential in the external medium by 210 mosmol caused a further increase in Cm by 26+/-6%. These data indicate two independent pathways for insertion of membrane into the plasma membrane. One is driven by Ca(i) and recruits membrane from a small pool. The osmotic evoked rise in surface area draws membrane from a much larger reservoir and may be driven by membrane tension. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_botanik ?? 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) 10 Fachbereich Biologie > Pflanzliche Zellbiologie |
| Hinterlegungsdatum: | 22 Jun 2011 07:39 |
| Letzte Änderung: | 05 Mär 2013 09:49 |
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