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Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cells

Brzostowski, Joseph A. and Meckel, Tobias and Hong, Jiang and Chen, Alice and Jin, Tian (2009):
Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cells.
In: Current Protocols in Protein Science, 56 (1), pp. 19.5.1-19.5.12. Wiley, ISSN 1934-3655,
DOI: 10.1002/0471140864.ps1905s56,
[Article]

Abstract

This unit describes an acceptor-sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acceptor photobleaching, which is an end-point assay that destroys the acceptor fluorophore, the sensitized emission method is amenable for FRET measurements in live cells and can be used to measure changes in FRET efficiency over time. The purpose of this unit is to provide a basic starting point for understanding and performing the sensitized emission method with a simple teaching tool for live-cell imaging. References that discuss the vagaries of acquiring and analyzing FRET between individually tagged molecules are provided.

Item Type: Article
Erschienen: 2009
Creators: Brzostowski, Joseph A. and Meckel, Tobias and Hong, Jiang and Chen, Alice and Jin, Tian
Title: Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cells
Language: English
Abstract:

This unit describes an acceptor-sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acceptor photobleaching, which is an end-point assay that destroys the acceptor fluorophore, the sensitized emission method is amenable for FRET measurements in live cells and can be used to measure changes in FRET efficiency over time. The purpose of this unit is to provide a basic starting point for understanding and performing the sensitized emission method with a simple teaching tool for live-cell imaging. References that discuss the vagaries of acquiring and analyzing FRET between individually tagged molecules are provided.

Journal or Publication Title: Current Protocols in Protein Science
Journal volume: 56
Number: 1
Publisher: Wiley
Divisions: 10 Department of Biology
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10 Department of Biology > Plant Membrane Biophysics
10 Department of Biology > Membrane Dynamics
Date Deposited: 06 Jun 2011 13:32
DOI: 10.1002/0471140864.ps1905s56
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