Brzostowski, Joseph A. ; Meckel, Tobias ; Hong, Jiang ; Chen, Alice ; Jin, Tian (2009)
Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cells.
In: Current Protocols in Protein Science, 56 (1)
doi: 10.1002/0471140864.ps1905s56
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
This unit describes an acceptor-sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acceptor photobleaching, which is an end-point assay that destroys the acceptor fluorophore, the sensitized emission method is amenable for FRET measurements in live cells and can be used to measure changes in FRET efficiency over time. The purpose of this unit is to provide a basic starting point for understanding and performing the sensitized emission method with a simple teaching tool for live-cell imaging. References that discuss the vagaries of acquiring and analyzing FRET between individually tagged molecules are provided.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2009 |
| Autor(en): | Brzostowski, Joseph A. ; Meckel, Tobias ; Hong, Jiang ; Chen, Alice ; Jin, Tian |
| Art des Eintrags: | Bibliographie |
| Titel: | Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cells |
| Sprache: | Englisch |
| Publikationsjahr: | 1 April 2009 |
| Verlag: | Wiley |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Current Protocols in Protein Science |
| Jahrgang/Volume einer Zeitschrift: | 56 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.1002/0471140864.ps1905s56 |
| Kurzbeschreibung (Abstract): | This unit describes an acceptor-sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acceptor photobleaching, which is an end-point assay that destroys the acceptor fluorophore, the sensitized emission method is amenable for FRET measurements in live cells and can be used to measure changes in FRET efficiency over time. The purpose of this unit is to provide a basic starting point for understanding and performing the sensitized emission method with a simple teaching tool for live-cell imaging. References that discuss the vagaries of acquiring and analyzing FRET between individually tagged molecules are provided. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_botanik ?? 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) 10 Fachbereich Biologie > Membrane Dynamics |
| Hinterlegungsdatum: | 06 Jun 2011 13:32 |
| Letzte Änderung: | 16 Jun 2020 11:20 |
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