Liu, Wanli ; Meckel, Tobias ; Tolar, Pavel ; Sohn, Hae Won ; Pierce, Susan K. (2010)
Antigen affinity discrimination is an intrinsic function of the B cell receptor.
In: The Journal of experimental medicine, 207 (5)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Antibody affinity maturation, a hallmark of adaptive immune responses, results from the selection of B cells expressing somatically hypermutated B cell receptors (BCRs) with increased affinity for antigens. Despite the central role of affinity maturation in antibody responses, the molecular mechanisms by which the increased affinity of a B cell for antigen is translated into a selective advantage for that B cell in immune responses is incompletely understood. We use high resolution live-cell imaging to provide evidence that the earliest BCR-intrinsic events that follow within seconds of BCR-antigen binding are highly sensitive to the affinity of the BCR for antigen. High affinity BCRs readily form oligomers and the resulting microclusters grow rapidly, resulting in enhanced recruitment of Syk kinase and calcium fluxes. Thus, B cells are able to read the affinity of antigen by BCR-intrinsic mechanisms during the earliest phases of BCR clustering, leading to the initiation of B cell responses.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2010 |
| Autor(en): | Liu, Wanli ; Meckel, Tobias ; Tolar, Pavel ; Sohn, Hae Won ; Pierce, Susan K. |
| Art des Eintrags: | Bibliographie |
| Titel: | Antigen affinity discrimination is an intrinsic function of the B cell receptor. |
| Sprache: | Englisch |
| Publikationsjahr: | 2010 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | The Journal of experimental medicine |
| Jahrgang/Volume einer Zeitschrift: | 207 |
| (Heft-)Nummer: | 5 |
| Kurzbeschreibung (Abstract): | Antibody affinity maturation, a hallmark of adaptive immune responses, results from the selection of B cells expressing somatically hypermutated B cell receptors (BCRs) with increased affinity for antigens. Despite the central role of affinity maturation in antibody responses, the molecular mechanisms by which the increased affinity of a B cell for antigen is translated into a selective advantage for that B cell in immune responses is incompletely understood. We use high resolution live-cell imaging to provide evidence that the earliest BCR-intrinsic events that follow within seconds of BCR-antigen binding are highly sensitive to the affinity of the BCR for antigen. High affinity BCRs readily form oligomers and the resulting microclusters grow rapidly, resulting in enhanced recruitment of Syk kinase and calcium fluxes. Thus, B cells are able to read the affinity of antigen by BCR-intrinsic mechanisms during the earliest phases of BCR clustering, leading to the initiation of B cell responses. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_botanik ?? 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) 10 Fachbereich Biologie > Membrane Dynamics |
| Hinterlegungsdatum: | 06 Jun 2011 13:10 |
| Letzte Änderung: | 05 Mär 2013 09:48 |
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