Boesler, C. ; Kruse, J. ; Söderbom, F. ; Hammann, Christian (2011)
Sequence and generation of mature ribosomal RNA transcripts in Dictyostelium discoideum.
In: The Journal of biological chemistry, 286 (20)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The amoeba Dictyostelium discoideum is a well-established model organism for studying numerous aspects of cellular and developmental functions. Its ribosomal RNA (rRNA) is encoded in an extrachromosomal palindrome that exists in ca. 100 copies in the cell. In this study, we have set out investigate the sequence of the expressed rRNA. For this, we have ligated the rRNA ends and performed RT-PCR on these circular RNAs. Sequencing revealed that the mature 26S, 17S, 5.8S and 5S rRNAs have sizes of 3741, 1871, 162 and 112 nucleotides, respectively. Unlike published, all mature rRNAs of a type uniformly display the same start and end nucleotides in the analyzed AX2 strain. We show the existence of a short-lived primary transcript covering the rRNA transcription unit of 17S, 5.8S and 26S rRNA. Northern blots and RT-PCR reveal that from this primary transcript two precursor molecules of the 17S and two precursors of the 26S rRNA are generated. We have also determined the sequences of these precursor molecules, and based on this data, we propose a model for the maturation of the rRNAs in Dictyostelium discoideum that we compare to the processing of the rRNA transcription unit of Saccharomyces cerevisiae.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2011 |
| Autor(en): | Boesler, C. ; Kruse, J. ; Söderbom, F. ; Hammann, Christian |
| Art des Eintrags: | Bibliographie |
| Titel: | Sequence and generation of mature ribosomal RNA transcripts in Dictyostelium discoideum. |
| Sprache: | Englisch |
| Publikationsjahr: | 2011 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | The Journal of biological chemistry |
| Jahrgang/Volume einer Zeitschrift: | 286 |
| (Heft-)Nummer: | 20 |
| Kurzbeschreibung (Abstract): | The amoeba Dictyostelium discoideum is a well-established model organism for studying numerous aspects of cellular and developmental functions. Its ribosomal RNA (rRNA) is encoded in an extrachromosomal palindrome that exists in ca. 100 copies in the cell. In this study, we have set out investigate the sequence of the expressed rRNA. For this, we have ligated the rRNA ends and performed RT-PCR on these circular RNAs. Sequencing revealed that the mature 26S, 17S, 5.8S and 5S rRNAs have sizes of 3741, 1871, 162 and 112 nucleotides, respectively. Unlike published, all mature rRNAs of a type uniformly display the same start and end nucleotides in the analyzed AX2 strain. We show the existence of a short-lived primary transcript covering the rRNA transcription unit of 17S, 5.8S and 26S rRNA. Northern blots and RT-PCR reveal that from this primary transcript two precursor molecules of the 17S and two precursors of the 26S rRNA are generated. We have also determined the sequences of these precursor molecules, and based on this data, we propose a model for the maturation of the rRNAs in Dictyostelium discoideum that we compare to the processing of the rRNA transcription unit of Saccharomyces cerevisiae. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Regulatorische RNAs und Ribozyme ?? fb10_mikrobiologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 05 Apr 2011 06:33 |
| Letzte Änderung: | 05 Mär 2013 09:47 |
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