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Hydrogen Peroxide Sensing with Horseradish Peroxidase-Modified Polymer Single Conical Nanochannels

Ali, Mubarak and Tahir, Muhammad Nawaz and Siwy, Zuzanna and Neumann, Reinhard and Tremel, Wolfgang and Ensinger, Wolfgang (2011):
Hydrogen Peroxide Sensing with Horseradish Peroxidase-Modified Polymer Single Conical Nanochannels.
83, In: Analytical Chemistry, (5), ACS Publications, pp. 1673-1680, [Online-Edition: http://dx.doi.org/10.1021/ac102795a],
[Article]

Abstract

Inspired from the funtioning and responsiveness of biological ion channels, researchers attempt to develop biosensing systems based on polymer and solid-state nanochannels. The applicability of these nanochannels for detection/sensing of any foreign analyte in the surrounding environment depends critically on the surface characteristics of the inner walls. Attaching recognition sites to the channel walls leads to the preparation of sensors targeted at a specific molecule. There are many nanochannel platforms for the detection of DNA and proteins, but only a few are capable of detecting small molecules. Here, we describe a nanochannel platform for the detection of hydrogen peroxide, H2O2, which is not only a toxic waste product in the cellular systems but also a key player in the redox signaling pathways. The sensor is based on single conical nanochannels fabricated in an ion tracked polymer membrane. The inner walls of the channel are decorated with horseradish peroxidase (HRP) enzyme using carbodiimide coupling chemistry. The success of the HRP immobilization on the channel surface is confirmed by measuring the pH-dependent current-voltage (I-V) curves of the system. The reported HRP-nanochannel system detects nanomolar concentrations of H2O2 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as the substrate. The immobilized HRP enzyme is thus capable of inducing redox reactions in a subfemtoliter volume of single nanochannels. We demonstrate that functioning of the designed biosensor is reversible and can be used multiple times to detect H2O2 at various concentrations.

Item Type: Article
Erschienen: 2011
Creators: Ali, Mubarak and Tahir, Muhammad Nawaz and Siwy, Zuzanna and Neumann, Reinhard and Tremel, Wolfgang and Ensinger, Wolfgang
Title: Hydrogen Peroxide Sensing with Horseradish Peroxidase-Modified Polymer Single Conical Nanochannels
Language: English
Abstract:

Inspired from the funtioning and responsiveness of biological ion channels, researchers attempt to develop biosensing systems based on polymer and solid-state nanochannels. The applicability of these nanochannels for detection/sensing of any foreign analyte in the surrounding environment depends critically on the surface characteristics of the inner walls. Attaching recognition sites to the channel walls leads to the preparation of sensors targeted at a specific molecule. There are many nanochannel platforms for the detection of DNA and proteins, but only a few are capable of detecting small molecules. Here, we describe a nanochannel platform for the detection of hydrogen peroxide, H2O2, which is not only a toxic waste product in the cellular systems but also a key player in the redox signaling pathways. The sensor is based on single conical nanochannels fabricated in an ion tracked polymer membrane. The inner walls of the channel are decorated with horseradish peroxidase (HRP) enzyme using carbodiimide coupling chemistry. The success of the HRP immobilization on the channel surface is confirmed by measuring the pH-dependent current-voltage (I-V) curves of the system. The reported HRP-nanochannel system detects nanomolar concentrations of H2O2 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as the substrate. The immobilized HRP enzyme is thus capable of inducing redox reactions in a subfemtoliter volume of single nanochannels. We demonstrate that functioning of the designed biosensor is reversible and can be used multiple times to detect H2O2 at various concentrations.

Journal or Publication Title: Analytical Chemistry
Volume: 83
Number: 5
Publisher: ACS Publications
Divisions: 11 Department of Materials and Earth Sciences > Material Science > Material Analytics
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences
Date Deposited: 24 Mar 2011 13:17
Official URL: http://dx.doi.org/10.1021/ac102795a
Funders: M.A., R.N., and W.E. gratefully acknowledge financial support by the Beilstein-Institut, Frankfurt/Main, Germany, within the research collaboration NanoBiC., Z.S. is grateful to the Alexander von Humboldt Foundation for the Friedrich Wilhelm Bessel Award and the National Science Foundation (CHE 0747237, CMMI 825661)., M.N.T and W.T. are thankful to the Deutsche Forschungsgemeinschaft, the Bundesministerium für Bildung und Forschung, Germany [Center of Excellence BIOTECmarin], the European Society for Marine Biotechnology,, and the International Human Frontier Science Program for their financial support.
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