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Hydrogen Peroxide Sensing with Horseradish Peroxidase-Modified Polymer Single Conical Nanochannels

Ali, Mubarak ; Tahir, Muhammad Nawaz ; Siwy, Zuzanna ; Neumann, Reinhard ; Tremel, Wolfgang ; Ensinger, Wolfgang :
Hydrogen Peroxide Sensing with Horseradish Peroxidase-Modified Polymer Single Conical Nanochannels.
[Online-Edition: http://dx.doi.org/10.1021/ac102795a]
In: Analytical Chemistry, 83 (5) pp. 1673-1680.
[Artikel], (2011)

Offizielle URL: http://dx.doi.org/10.1021/ac102795a

Kurzbeschreibung (Abstract)

Inspired from the funtioning and responsiveness of biological ion channels, researchers attempt to develop biosensing systems based on polymer and solid-state nanochannels. The applicability of these nanochannels for detection/sensing of any foreign analyte in the surrounding environment depends critically on the surface characteristics of the inner walls. Attaching recognition sites to the channel walls leads to the preparation of sensors targeted at a specific molecule. There are many nanochannel platforms for the detection of DNA and proteins, but only a few are capable of detecting small molecules. Here, we describe a nanochannel platform for the detection of hydrogen peroxide, H2O2, which is not only a toxic waste product in the cellular systems but also a key player in the redox signaling pathways. The sensor is based on single conical nanochannels fabricated in an ion tracked polymer membrane. The inner walls of the channel are decorated with horseradish peroxidase (HRP) enzyme using carbodiimide coupling chemistry. The success of the HRP immobilization on the channel surface is confirmed by measuring the pH-dependent current-voltage (I-V) curves of the system. The reported HRP-nanochannel system detects nanomolar concentrations of H2O2 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as the substrate. The immobilized HRP enzyme is thus capable of inducing redox reactions in a subfemtoliter volume of single nanochannels. We demonstrate that functioning of the designed biosensor is reversible and can be used multiple times to detect H2O2 at various concentrations.

Typ des Eintrags: Artikel
Erschienen: 2011
Autor(en): Ali, Mubarak ; Tahir, Muhammad Nawaz ; Siwy, Zuzanna ; Neumann, Reinhard ; Tremel, Wolfgang ; Ensinger, Wolfgang
Titel: Hydrogen Peroxide Sensing with Horseradish Peroxidase-Modified Polymer Single Conical Nanochannels
Sprache: Englisch
Kurzbeschreibung (Abstract):

Inspired from the funtioning and responsiveness of biological ion channels, researchers attempt to develop biosensing systems based on polymer and solid-state nanochannels. The applicability of these nanochannels for detection/sensing of any foreign analyte in the surrounding environment depends critically on the surface characteristics of the inner walls. Attaching recognition sites to the channel walls leads to the preparation of sensors targeted at a specific molecule. There are many nanochannel platforms for the detection of DNA and proteins, but only a few are capable of detecting small molecules. Here, we describe a nanochannel platform for the detection of hydrogen peroxide, H2O2, which is not only a toxic waste product in the cellular systems but also a key player in the redox signaling pathways. The sensor is based on single conical nanochannels fabricated in an ion tracked polymer membrane. The inner walls of the channel are decorated with horseradish peroxidase (HRP) enzyme using carbodiimide coupling chemistry. The success of the HRP immobilization on the channel surface is confirmed by measuring the pH-dependent current-voltage (I-V) curves of the system. The reported HRP-nanochannel system detects nanomolar concentrations of H2O2 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as the substrate. The immobilized HRP enzyme is thus capable of inducing redox reactions in a subfemtoliter volume of single nanochannels. We demonstrate that functioning of the designed biosensor is reversible and can be used multiple times to detect H2O2 at various concentrations.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Analytical Chemistry
Band: 83
(Heft-)Nummer: 5
Verlag: ACS Publications
Fachbereich(e)/-gebiet(e): Fachbereich Material- und Geowissenschaften > Materialwissenschaften > Materialanalytik
Fachbereich Material- und Geowissenschaften > Materialwissenschaften
Fachbereich Material- und Geowissenschaften
Hinterlegungsdatum: 24 Mär 2011 13:17
Offizielle URL: http://dx.doi.org/10.1021/ac102795a
Sponsoren: M.A., R.N., and W.E. gratefully acknowledge financial support by the Beilstein-Institut, Frankfurt/Main, Germany, within the research collaboration NanoBiC., Z.S. is grateful to the Alexander von Humboldt Foundation for the Friedrich Wilhelm Bessel Award and the National Science Foundation (CHE 0747237, CMMI 825661)., M.N.T and W.T. are thankful to the Deutsche Forschungsgemeinschaft, the Bundesministerium für Bildung und Forschung, Germany [Center of Excellence BIOTECmarin], the European Society for Marine Biotechnology,, and the International Human Frontier Science Program for their financial support.
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