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Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid.

Holmes, M. and Pfeifer, Felicitas and Dyall-Smith, M. (1994):
Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid.
In: Gene, 146 (1), pp. 117-21. ISSN 0378-1119,
[Article]

Abstract

Two new Haloferax-Escherichia shuttle vectors are described, pMDS20 and pMLH3. These vectors contain the E. coli ColE1 plasmid ori region and ampicillin-resistance(ApR)-conferring bla gene, and the Haloferax pHK2 replicon region and novobiocin-resistance(NbR)-encoding gyrB gene, enabling maintenance and selection in both hosts. Plasmid pMLH3 has, in addition, a H. volcanii mevinolin-resistance (MvR) determinant and restriction sites allowing insertional inactivation of either marker, to facilitate the identification of Haloferax transformants harbouring cloned sequences. Sequencing of gyrA, within the NbR determinant, and the pHK2 ori region has been completed so the complete sequence of both pMDS20 and pMLH3 is now known.

Item Type: Article
Erschienen: 1994
Creators: Holmes, M. and Pfeifer, Felicitas and Dyall-Smith, M.
Title: Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid.
Language: English
Abstract:

Two new Haloferax-Escherichia shuttle vectors are described, pMDS20 and pMLH3. These vectors contain the E. coli ColE1 plasmid ori region and ampicillin-resistance(ApR)-conferring bla gene, and the Haloferax pHK2 replicon region and novobiocin-resistance(NbR)-encoding gyrB gene, enabling maintenance and selection in both hosts. Plasmid pMLH3 has, in addition, a H. volcanii mevinolin-resistance (MvR) determinant and restriction sites allowing insertional inactivation of either marker, to facilitate the identification of Haloferax transformants harbouring cloned sequences. Sequencing of gyrA, within the NbR determinant, and the pHK2 ori region has been completed so the complete sequence of both pMDS20 and pMLH3 is now known.

Journal or Publication Title: Gene
Journal volume: 146
Number: 1
Divisions: 10 Department of Biology > Microbiology and Archaea
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10 Department of Biology
Date Deposited: 14 Feb 2011 10:49
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