Holmes, M. ; Pfeifer, Felicitas ; Dyall-Smith, M. (1994)
Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid.
In: Gene, 146 (1)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Two new Haloferax-Escherichia shuttle vectors are described, pMDS20 and pMLH3. These vectors contain the E. coli ColE1 plasmid ori region and ampicillin-resistance(ApR)-conferring bla gene, and the Haloferax pHK2 replicon region and novobiocin-resistance(NbR)-encoding gyrB gene, enabling maintenance and selection in both hosts. Plasmid pMLH3 has, in addition, a H. volcanii mevinolin-resistance (MvR) determinant and restriction sites allowing insertional inactivation of either marker, to facilitate the identification of Haloferax transformants harbouring cloned sequences. Sequencing of gyrA, within the NbR determinant, and the pHK2 ori region has been completed so the complete sequence of both pMDS20 and pMLH3 is now known.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1994 |
| Autor(en): | Holmes, M. ; Pfeifer, Felicitas ; Dyall-Smith, M. |
| Art des Eintrags: | Bibliographie |
| Titel: | Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid. |
| Sprache: | Englisch |
| Publikationsjahr: | 1994 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Gene |
| Jahrgang/Volume einer Zeitschrift: | 146 |
| (Heft-)Nummer: | 1 |
| Kurzbeschreibung (Abstract): | Two new Haloferax-Escherichia shuttle vectors are described, pMDS20 and pMLH3. These vectors contain the E. coli ColE1 plasmid ori region and ampicillin-resistance(ApR)-conferring bla gene, and the Haloferax pHK2 replicon region and novobiocin-resistance(NbR)-encoding gyrB gene, enabling maintenance and selection in both hosts. Plasmid pMLH3 has, in addition, a H. volcanii mevinolin-resistance (MvR) determinant and restriction sites allowing insertional inactivation of either marker, to facilitate the identification of Haloferax transformants harbouring cloned sequences. Sequencing of gyrA, within the NbR determinant, and the pHK2 ori region has been completed so the complete sequence of both pMDS20 and pMLH3 is now known. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Microbiology and Archaea ?? fb10_mikrobiologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 14 Feb 2011 10:49 |
| Letzte Änderung: | 05 Mär 2013 09:45 |
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