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Fumarate respiration of Wolinella succinogenes: enzymology, energetics and coupling mechanism.

Kröger, A. and Biel, S. and Simon, J. and Gross, R. and Unden, G. and Lancaster, C. R. D. (2002):
Fumarate respiration of Wolinella succinogenes: enzymology, energetics and coupling mechanism.
In: Biochimica et biophysica acta, pp. 23-38, 1553, (1-2), ISSN 0006-3002,
[Article]

Abstract

Wolinella succinogenes performs oxidative phosphorylation with fumarate instead of O2 as terminal electron acceptor and H2 or formate as electron donors. Fumarate reduction by these donors ('fumarate respiration') is catalyzed by an electron transport chain in the bacterial membrane, and is coupled to the generation of an electrochemical proton potential (Deltap) across the bacterial membrane. The experimental evidence concerning the electron transport and its coupling to Deltap generation is reviewed in this article. The electron transport chain consists of fumarate reductase, menaquinone (MK) and either hydrogenase or formate dehydrogenase. Measurements indicate that the Deltap is generated exclusively by MK reduction with H2 or formate; MKH2 oxidation by fumarate appears to be an electroneutral process. However, evidence derived from the crystal structure of fumarate reductase suggests an electrogenic mechanism for the latter process.

Item Type: Article
Erschienen: 2002
Creators: Kröger, A. and Biel, S. and Simon, J. and Gross, R. and Unden, G. and Lancaster, C. R. D.
Title: Fumarate respiration of Wolinella succinogenes: enzymology, energetics and coupling mechanism.
Language: English
Abstract:

Wolinella succinogenes performs oxidative phosphorylation with fumarate instead of O2 as terminal electron acceptor and H2 or formate as electron donors. Fumarate reduction by these donors ('fumarate respiration') is catalyzed by an electron transport chain in the bacterial membrane, and is coupled to the generation of an electrochemical proton potential (Deltap) across the bacterial membrane. The experimental evidence concerning the electron transport and its coupling to Deltap generation is reviewed in this article. The electron transport chain consists of fumarate reductase, menaquinone (MK) and either hydrogenase or formate dehydrogenase. Measurements indicate that the Deltap is generated exclusively by MK reduction with H2 or formate; MKH2 oxidation by fumarate appears to be an electroneutral process. However, evidence derived from the crystal structure of fumarate reductase suggests an electrogenic mechanism for the latter process.

Journal or Publication Title: Biochimica et biophysica acta
Volume: 1553
Number: 1-2
Divisions: 10 Department of Biology > Microbial Energy Conversion and Biotechnology
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10 Department of Biology
Date Deposited: 07 Dec 2010 15:11
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