Bertl, A. ; Anderson, J. A. ; Slayman, C. L. ; Sentenac, H. ; Gaber, R. F. (1994)
Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.
In: Folia microbiologica, 39 (6)
doi: 10.1007/BF02814074
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Disruption of genes encoding endogenous transport proteins in Saccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters from the plant Arabidopsis thaliana [1-4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of the trk1deltatrk2delta double-deletion mutant with KAT1 from Arabidopsis thaliana restores this phenotype by encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of a trk1 mutant with AKT1 from A. thaliana.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1994 |
| Autor(en): | Bertl, A. ; Anderson, J. A. ; Slayman, C. L. ; Sentenac, H. ; Gaber, R. F. |
| Art des Eintrags: | Bibliographie |
| Titel: | Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels. |
| Sprache: | Englisch |
| Publikationsjahr: | 1994 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Folia microbiologica |
| Jahrgang/Volume einer Zeitschrift: | 39 |
| (Heft-)Nummer: | 6 |
| DOI: | 10.1007/BF02814074 |
| Kurzbeschreibung (Abstract): | Disruption of genes encoding endogenous transport proteins in Saccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters from the plant Arabidopsis thaliana [1-4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of the trk1deltatrk2delta double-deletion mutant with KAT1 from Arabidopsis thaliana restores this phenotype by encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of a trk1 mutant with AKT1 from A. thaliana. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_botanik ?? 10 Fachbereich Biologie > Yeast Membrane Biology |
| Hinterlegungsdatum: | 25 Nov 2010 15:29 |
| Letzte Änderung: | 09 Mär 2022 12:51 |
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