Porée, F. ; Wulfetange, K. ; Naso, A. ; Carpaneto, A. ; Roller, A. ; Natura, G. ; Bertl, A. ; Sentenac, H. ; Thibaud, J. ; Dreyer, I. (2005)
Plant K(in) and K(out) channels: approaching the trait of opposite rectification by analyzing more than 250 KAT1-SKOR chimeras.
In: Biochemical and biophysical research communications, 332 (2)
doi: 10.1016/j.bbrc.2005.04.150
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Members of the Shaker-like plant K(+) channel family share a common structure, but are highly diverse in their function: they behave as either hyperpolarization-activated inward-rectifying (K(in)) channels, or leak-like (K(weak)) channels, or depolarization-activated outward-rectifying (K(out)) channels. Here we created 256 chimeras between the K(in) channel KAT1 and the K(out) channel SKOR. The chimeras were screened in a potassium-uptake deficient yeast strain to identify those, which mediate potassium inward currents, i.e., which are functionally equivalent to KAT1. This strategy allowed us to identify three chimeras which differ from KAT1 in three parts of the polypeptide: the cytosolic N-terminus, the cytosolic C-terminus, and the putative voltage-sensor S4. Additionally, mutations in the K(out) channel SKOR were generated in order to localize molecular entities underlying its depolarization activation. The triple mutant SKOR-D312N-M313L-I314G, carrying amino-acid changes in the S6 segment, was identified as a channel which did not display any rectification in the tested voltage-range.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2005 |
| Autor(en): | Porée, F. ; Wulfetange, K. ; Naso, A. ; Carpaneto, A. ; Roller, A. ; Natura, G. ; Bertl, A. ; Sentenac, H. ; Thibaud, J. ; Dreyer, I. |
| Art des Eintrags: | Bibliographie |
| Titel: | Plant K(in) and K(out) channels: approaching the trait of opposite rectification by analyzing more than 250 KAT1-SKOR chimeras. |
| Sprache: | Englisch |
| Publikationsjahr: | 2005 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biochemical and biophysical research communications |
| Jahrgang/Volume einer Zeitschrift: | 332 |
| (Heft-)Nummer: | 2 |
| DOI: | 10.1016/j.bbrc.2005.04.150 |
| Kurzbeschreibung (Abstract): | Members of the Shaker-like plant K(+) channel family share a common structure, but are highly diverse in their function: they behave as either hyperpolarization-activated inward-rectifying (K(in)) channels, or leak-like (K(weak)) channels, or depolarization-activated outward-rectifying (K(out)) channels. Here we created 256 chimeras between the K(in) channel KAT1 and the K(out) channel SKOR. The chimeras were screened in a potassium-uptake deficient yeast strain to identify those, which mediate potassium inward currents, i.e., which are functionally equivalent to KAT1. This strategy allowed us to identify three chimeras which differ from KAT1 in three parts of the polypeptide: the cytosolic N-terminus, the cytosolic C-terminus, and the putative voltage-sensor S4. Additionally, mutations in the K(out) channel SKOR were generated in order to localize molecular entities underlying its depolarization activation. The triple mutant SKOR-D312N-M313L-I314G, carrying amino-acid changes in the S6 segment, was identified as a channel which did not display any rectification in the tested voltage-range. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_botanik ?? 10 Fachbereich Biologie > Yeast Membrane Biology |
| Hinterlegungsdatum: | 29 Nov 2010 15:29 |
| Letzte Änderung: | 05 Mär 2013 09:41 |
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