Gehwolf, R. ; Weiss, R. ; Gabler, M. ; Hurst, A. C. ; Bertl, A. ; Thalhamer, J. ; Obermeyer, G. (2007)
From sequence to antibody: genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel.
In: FEBS letters, 581 (3)
doi: 10.1016/j.febslet.2007.01.004
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Monoclonal antibodies against the K(+) channel KAT1 of Arabidopsis thaliana, a low abundance, plant plasma membrane protein, were generated by genetic immunisation to avoid the time and labour consuming purification of native or recombinant proteins and peptides usually necessary for conventional immunisation techniques. The resulting polyclonal and monoclonal antibody sera recognised a single protein band in a microsomal fraction of wild-type A. thaliana leaves and in membrane fractions of transgenic yeast cells and tobacco plants expressing the KAT1 protein. Therefore, genetic immunisation is suitable for generating monoclonal antibodies against plant proteins and particularly, against plant membrane proteins of low abundance.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2007 |
| Autor(en): | Gehwolf, R. ; Weiss, R. ; Gabler, M. ; Hurst, A. C. ; Bertl, A. ; Thalhamer, J. ; Obermeyer, G. |
| Art des Eintrags: | Bibliographie |
| Titel: | From sequence to antibody: genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel. |
| Sprache: | Englisch |
| Publikationsjahr: | 2007 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | FEBS letters |
| Jahrgang/Volume einer Zeitschrift: | 581 |
| (Heft-)Nummer: | 3 |
| DOI: | 10.1016/j.febslet.2007.01.004 |
| Kurzbeschreibung (Abstract): | Monoclonal antibodies against the K(+) channel KAT1 of Arabidopsis thaliana, a low abundance, plant plasma membrane protein, were generated by genetic immunisation to avoid the time and labour consuming purification of native or recombinant proteins and peptides usually necessary for conventional immunisation techniques. The resulting polyclonal and monoclonal antibody sera recognised a single protein band in a microsomal fraction of wild-type A. thaliana leaves and in membrane fractions of transgenic yeast cells and tobacco plants expressing the KAT1 protein. Therefore, genetic immunisation is suitable for generating monoclonal antibodies against plant proteins and particularly, against plant membrane proteins of low abundance. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_botanik ?? 10 Fachbereich Biologie > Yeast Membrane Biology |
| Hinterlegungsdatum: | 29 Nov 2010 15:31 |
| Letzte Änderung: | 05 Mär 2013 09:41 |
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