Hammann, Christian ; Lilley, David M. J. (2002)
Folding and activity of the hammerhead ribozyme.
In: Chembiochem : a European journal of chemical biology, 3 (8)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The hammerhead is the smallest of the nucleolytic ribozymes, that undergo backbone cleavage by a transesterification reaction in the presence of magnesium ions. The RNA is induced to fold into its active conformation by the binding of metal ions in two stages. These generate domain 2, the scaffold on which the ribozyme is built, and domain 1, the active centre of the ribozyme. Further local structural rearrangement during the activation of the ribozyme is suggested by a number of crystal structures. The 10(5)-fold rate enhancement is probably brought about by a combination of metal-ion participation and stereochemical factors in the environment of the folded RNA structure.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2002 |
| Autor(en): | Hammann, Christian ; Lilley, David M. J. |
| Art des Eintrags: | Bibliographie |
| Titel: | Folding and activity of the hammerhead ribozyme. |
| Sprache: | Englisch |
| Publikationsjahr: | 2002 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Chembiochem : a European journal of chemical biology |
| Jahrgang/Volume einer Zeitschrift: | 3 |
| (Heft-)Nummer: | 8 |
| Kurzbeschreibung (Abstract): | The hammerhead is the smallest of the nucleolytic ribozymes, that undergo backbone cleavage by a transesterification reaction in the presence of magnesium ions. The RNA is induced to fold into its active conformation by the binding of metal ions in two stages. These generate domain 2, the scaffold on which the ribozyme is built, and domain 1, the active centre of the ribozyme. Further local structural rearrangement during the activation of the ribozyme is suggested by a number of crystal structures. The 10(5)-fold rate enhancement is probably brought about by a combination of metal-ion participation and stereochemical factors in the environment of the folded RNA structure. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_mikrobiologie ?? 10 Fachbereich Biologie > Regulatorische RNAs und Ribozyme |
| Hinterlegungsdatum: | 29 Jul 2010 12:20 |
| Letzte Änderung: | 05 Mär 2013 09:34 |
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