Görisch, Sabine M. ; Richter, Karsten ; Scheuermann, Markus O. ; Herrmann, Harald ; Lichter, Peter (2003)
Diffusion-limited compartmentalization of mammalian cell nuclei assessed by microinjected macromolecules.
In: Experimental cell research, 289 (2)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
In order to investigate the accessibility of the nucleoplasm for macromolecules with different physical properties, we microinjected FITC-conjugated dextrans of different sizes as well as anionic FITC-dextrans and FITC-poly-L-lysine into mammalian cell nuclei. Small dextrans displayed a homogeneous nuclear distribution. With increasing molecular mass (42 to 2500 kDa), FITC-dextrans were progressively excluded from chromatin regions, accumulating in and thereby outlining an apparently extended interchromatin space. Anionic FITC-dextrans (500 kDa) showed complete exclusion from labeled chromatin regions, while the positively charged FITC-poly-L-lysine was to some extent present within the chromatin regions. Moreover, the FITC-poly-L-lysine preferentially localized at the nuclear periphery. We also found a size-dependent exclusion of FITC-dextrans from nucleoli regions, while the FITC-poly-L-lysine accumulated in the nucleoli. Thus, the distinct and restricted nuclear accessibility for macromolecules is dependent on molecule size and electrical charge.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2003 |
| Autor(en): | Görisch, Sabine M. ; Richter, Karsten ; Scheuermann, Markus O. ; Herrmann, Harald ; Lichter, Peter |
| Art des Eintrags: | Bibliographie |
| Titel: | Diffusion-limited compartmentalization of mammalian cell nuclei assessed by microinjected macromolecules. |
| Sprache: | Deutsch |
| Publikationsjahr: | 2003 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Experimental cell research |
| Jahrgang/Volume einer Zeitschrift: | 289 |
| (Heft-)Nummer: | 2 |
| URL / URN: | http://www.cardoso-lab.org/publications/Goerisch_2003.pdf |
| Kurzbeschreibung (Abstract): | In order to investigate the accessibility of the nucleoplasm for macromolecules with different physical properties, we microinjected FITC-conjugated dextrans of different sizes as well as anionic FITC-dextrans and FITC-poly-L-lysine into mammalian cell nuclei. Small dextrans displayed a homogeneous nuclear distribution. With increasing molecular mass (42 to 2500 kDa), FITC-dextrans were progressively excluded from chromatin regions, accumulating in and thereby outlining an apparently extended interchromatin space. Anionic FITC-dextrans (500 kDa) showed complete exclusion from labeled chromatin regions, while the positively charged FITC-poly-L-lysine was to some extent present within the chromatin regions. Moreover, the FITC-poly-L-lysine preferentially localized at the nuclear periphery. We also found a size-dependent exclusion of FITC-dextrans from nucleoli regions, while the FITC-poly-L-lysine accumulated in the nucleoli. Thus, the distinct and restricted nuclear accessibility for macromolecules is dependent on molecule size and electrical charge. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 06 Mär 2010 07:32 |
| Letzte Änderung: | 05 Mär 2013 09:32 |
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