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Mobility of multi-subunit complexes in the nucleus: accessibility and dynamics of chromatin subcompartments.

Görisch, Sabine M. and Lichter, Peter and Rippe, Karsten :
Mobility of multi-subunit complexes in the nucleus: accessibility and dynamics of chromatin subcompartments.
[Online-Edition: http://www.cardoso-lab.org/publications/Goerisch_2005a.pdf]
In: Histochemistry and cell biology, 123 (3) pp. 217-28. ISSN 0948-6143
[Article] , (2005)

Official URL: http://www.cardoso-lab.org/publications/Goerisch_2005a.pdf

Abstract

The cell nucleus contains a number of mobile subnuclear organelles involved in RNA processing, transcriptional regulation and antiviral defence like Cajal and promyelocytic leukaemia (PML) bodies. It remains an open question how these bodies translocate to specific nuclear regions within the nucleus to exert their biological function. The mobility and localisation of macromolecules in the nucleus are closely related to the dynamic organisation and accessibility of chromatin. This relation has been studied with biologically inert fluorescent particles like dextrans, polystyrene nanospheres and inactive protein crystals formed by the Mx1-YFP fusion protein or other ectopically expressed proteins like vimentin. As reviewed here, properties of the chromatin environment can be identified from these experiments that determine the mobility of Cajal and PML bodies and other supramolecular complexes.

Item Type: Article
Erschienen: 2005
Creators: Görisch, Sabine M. and Lichter, Peter and Rippe, Karsten
Title: Mobility of multi-subunit complexes in the nucleus: accessibility and dynamics of chromatin subcompartments.
Language: German
Abstract:

The cell nucleus contains a number of mobile subnuclear organelles involved in RNA processing, transcriptional regulation and antiviral defence like Cajal and promyelocytic leukaemia (PML) bodies. It remains an open question how these bodies translocate to specific nuclear regions within the nucleus to exert their biological function. The mobility and localisation of macromolecules in the nucleus are closely related to the dynamic organisation and accessibility of chromatin. This relation has been studied with biologically inert fluorescent particles like dextrans, polystyrene nanospheres and inactive protein crystals formed by the Mx1-YFP fusion protein or other ectopically expressed proteins like vimentin. As reviewed here, properties of the chromatin environment can be identified from these experiments that determine the mobility of Cajal and PML bodies and other supramolecular complexes.

Journal or Publication Title: Histochemistry and cell biology
Volume: 123
Number: 3
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 06 Mar 2010 07:52
Official URL: http://www.cardoso-lab.org/publications/Goerisch_2005a.pdf
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