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Biosensing with Functionalized Single Asymmetric Polymer Nanochannels

Ali, Mubarak and Schiedt, Birgitta and Neumann, Reinhard and Ensinger, Wolfgang (2010):
Biosensing with Functionalized Single Asymmetric Polymer Nanochannels.
In: Macromolecular Bioscience, (1), 10. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, pp. 28-32, [Online-Edition: http://dx.doi.org/10.1002/mabi.200900198],
[Article]

Abstract

In this work, we describe the direct covalent attachment of protein recognition elements (biotin) with the carboxyl groups present on the walls of polyimide nanochannels. Subsequently, these biotinylated channels were used for the bio-specific sensing of protein analytes. Moreover, surface charge of these asymmetric nanochannels was reversed from negative to positive via the conversion of carboxyl groups into terminated amino groups. The negatively charge (carboxylated) and positively charged (aminated) channels were further used for the electrochemical sensing of bovine serum albumin (BSA, pI = 4.7). These biorecognition events were assessed from the changes in the ionic current flowing through the nanochannel.

Item Type: Article
Erschienen: 2010
Creators: Ali, Mubarak and Schiedt, Birgitta and Neumann, Reinhard and Ensinger, Wolfgang
Title: Biosensing with Functionalized Single Asymmetric Polymer Nanochannels
Language: English
Abstract:

In this work, we describe the direct covalent attachment of protein recognition elements (biotin) with the carboxyl groups present on the walls of polyimide nanochannels. Subsequently, these biotinylated channels were used for the bio-specific sensing of protein analytes. Moreover, surface charge of these asymmetric nanochannels was reversed from negative to positive via the conversion of carboxyl groups into terminated amino groups. The negatively charge (carboxylated) and positively charged (aminated) channels were further used for the electrochemical sensing of bovine serum albumin (BSA, pI = 4.7). These biorecognition events were assessed from the changes in the ionic current flowing through the nanochannel.

Journal or Publication Title: Macromolecular Bioscience
Journal volume: 10
Number: 1
Publisher: WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Uncontrolled Keywords: functionalization of polymers; ion channels; membranes; nanotechnology; polyamides; sensors
Divisions: 11 Department of Materials and Earth Sciences > Material Science > Material Analytics
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences
Date Deposited: 05 Jan 2010 15:56
Official URL: http://dx.doi.org/10.1002/mabi.200900198
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