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Fabrication and functionalization of single asymmetric nanochannels for electrostatic/hydrophobic association of protein molecules

Ali, Mubarak and Bayer, Veronika and Schiedt, Birgitta and Neumann, Reinhard and Ensinger, Wolfgang :
Fabrication and functionalization of single asymmetric nanochannels for electrostatic/hydrophobic association of protein molecules.
[Online-Edition: http://www.iop.org/EJ/abstract/0957-4484/19/48/485711/]
In: Nanotechnology, 19 (48) p. 485711.
[Article] , (2008)

Official URL: http://www.iop.org/EJ/abstract/0957-4484/19/48/485711/

Abstract

We have developed a facile and reproducible method for surfactant-controlled track-etching and chemical functionalization of single asymmetric nanochannels in PET (polyethylene terephthalate) membranes. Carboxyl groups present on the channel surface were converted into pentafluorophenyl esters using EDC/PFP (N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/pentafluorophenol) coupling chemistry. The resulting amine-reactive esters were further covalently coupled with ethylenediamine or propylamine in order to manipulate the charge polarity and hydrophilicity of the nanochannels, respectively. Characterization of the modified channels was done by measuring their current-voltage (I-V) curves as well as their permselectivity before and after the chemical modification. The electrostatic/hydrophobic association of bovine serum albumin on the channel surface was observed through the change in rectification behaviour upon the variation of pH values.

Item Type: Article
Erschienen: 2008
Creators: Ali, Mubarak and Bayer, Veronika and Schiedt, Birgitta and Neumann, Reinhard and Ensinger, Wolfgang
Title: Fabrication and functionalization of single asymmetric nanochannels for electrostatic/hydrophobic association of protein molecules
Language: English
Abstract:

We have developed a facile and reproducible method for surfactant-controlled track-etching and chemical functionalization of single asymmetric nanochannels in PET (polyethylene terephthalate) membranes. Carboxyl groups present on the channel surface were converted into pentafluorophenyl esters using EDC/PFP (N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/pentafluorophenol) coupling chemistry. The resulting amine-reactive esters were further covalently coupled with ethylenediamine or propylamine in order to manipulate the charge polarity and hydrophilicity of the nanochannels, respectively. Characterization of the modified channels was done by measuring their current-voltage (I-V) curves as well as their permselectivity before and after the chemical modification. The electrostatic/hydrophobic association of bovine serum albumin on the channel surface was observed through the change in rectification behaviour upon the variation of pH values.

Journal or Publication Title: Nanotechnology
Volume: 19
Number: 48
Publisher: IOP Publishing
Divisions: 11 Department of Materials and Earth Sciences > Material Science > Material Analytics
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences
Date Deposited: 06 Jul 2009 13:54
Official URL: http://www.iop.org/EJ/abstract/0957-4484/19/48/485711/
Funders: MA acknowledges partial financial support from the Higher Education Commission (HEC) of Pakistan.
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