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Fabrication and functionalization of single asymmetric nanochannels for electrostatic/hydrophobic association of protein molecules

Ali, Mubarak ; Bayer, Veronika ; Schiedt, Birgitta ; Neumann, Reinhard ; Ensinger, Wolfgang :
Fabrication and functionalization of single asymmetric nanochannels for electrostatic/hydrophobic association of protein molecules.
[Online-Edition: http://www.iop.org/EJ/abstract/0957-4484/19/48/485711/]
In: Nanotechnology, 19 (48) S. 485711.
[Artikel] , (2008)

Offizielle URL: http://www.iop.org/EJ/abstract/0957-4484/19/48/485711/

Kurzbeschreibung (Abstract)

We have developed a facile and reproducible method for surfactant-controlled track-etching and chemical functionalization of single asymmetric nanochannels in PET (polyethylene terephthalate) membranes. Carboxyl groups present on the channel surface were converted into pentafluorophenyl esters using EDC/PFP (N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/pentafluorophenol) coupling chemistry. The resulting amine-reactive esters were further covalently coupled with ethylenediamine or propylamine in order to manipulate the charge polarity and hydrophilicity of the nanochannels, respectively. Characterization of the modified channels was done by measuring their current-voltage (I-V) curves as well as their permselectivity before and after the chemical modification. The electrostatic/hydrophobic association of bovine serum albumin on the channel surface was observed through the change in rectification behaviour upon the variation of pH values.

Typ des Eintrags: Artikel
Erschienen: 2008
Autor(en): Ali, Mubarak ; Bayer, Veronika ; Schiedt, Birgitta ; Neumann, Reinhard ; Ensinger, Wolfgang
Titel: Fabrication and functionalization of single asymmetric nanochannels for electrostatic/hydrophobic association of protein molecules
Sprache: Englisch
Kurzbeschreibung (Abstract):

We have developed a facile and reproducible method for surfactant-controlled track-etching and chemical functionalization of single asymmetric nanochannels in PET (polyethylene terephthalate) membranes. Carboxyl groups present on the channel surface were converted into pentafluorophenyl esters using EDC/PFP (N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/pentafluorophenol) coupling chemistry. The resulting amine-reactive esters were further covalently coupled with ethylenediamine or propylamine in order to manipulate the charge polarity and hydrophilicity of the nanochannels, respectively. Characterization of the modified channels was done by measuring their current-voltage (I-V) curves as well as their permselectivity before and after the chemical modification. The electrostatic/hydrophobic association of bovine serum albumin on the channel surface was observed through the change in rectification behaviour upon the variation of pH values.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Nanotechnology
Band: 19
(Heft-)Nummer: 48
Verlag: IOP Publishing
Fachbereich(e)/-gebiet(e): 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Materialanalytik
11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft
11 Fachbereich Material- und Geowissenschaften
Hinterlegungsdatum: 06 Jul 2009 13:54
Offizielle URL: http://www.iop.org/EJ/abstract/0957-4484/19/48/485711/
Sponsoren: MA acknowledges partial financial support from the Higher Education Commission (HEC) of Pakistan.
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