Becker, Andrea ; Canut, Herve ; Lüttge, Ulrich ; Maeshima, Masayoshi ; Marigo, Gerard ; Ratajczak, Rafael (1995)
Purification and immunological comparison of the tonoplast H+-pyrophosphatase from cells of Catharanthus roseus and leaves from Mesembryanthemum crystallinum performing C3-photosynthesis and the obligate CAM plant Kalanchoe daigremontiana.
In: Journal of plant physiology, 146 (1-2)
doi: 10.1016/S0176-1617(11)81972-3
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The tonoplast H+-pyrophosphatase (EC 3.6.1.1) from photosynthetically inactive cell cultures of the C3-plant Catharanthus roseus (L.) G. Don., and from leaves of the C3/CAM-intermediate plant Mesembryanthemum crystallinum L. in the C3-state and of the obligate CAM-plant Kalanchoë daigremontiana Hamet et Perrier de la Bâthie, was purified using Mono Q anion-exchange and Superose 6 size-exclusion fast protein liquid chromatography. The combination of both chromatographical techniques led to a single polypeptide with an apparent molecular mass of 72 kDa exhibiting K+-stimulated pyrophosphate hydrolysis activity in preparations from C. roseus and M. crystallinum and to two polypeptides (72 and 70 kDa) in hydrolytic active fractions from K. daigremontiana. These polypeptides cross-reacted with an antiserum against the tonoplast inorganic H+-pyrophosphatase of Vigna radiata L. cv. Wilczek. The possible identity of the 70 kDa polypeptide of K. daigremontiana is discussed.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1995 |
| Autor(en): | Becker, Andrea ; Canut, Herve ; Lüttge, Ulrich ; Maeshima, Masayoshi ; Marigo, Gerard ; Ratajczak, Rafael |
| Art des Eintrags: | Bibliographie |
| Titel: | Purification and immunological comparison of the tonoplast H+-pyrophosphatase from cells of Catharanthus roseus and leaves from Mesembryanthemum crystallinum performing C3-photosynthesis and the obligate CAM plant Kalanchoe daigremontiana |
| Sprache: | Englisch |
| Publikationsjahr: | 1 Mai 1995 |
| Verlag: | Elsevier |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of plant physiology |
| Jahrgang/Volume einer Zeitschrift: | 146 |
| (Heft-)Nummer: | 1-2 |
| DOI: | 10.1016/S0176-1617(11)81972-3 |
| Kurzbeschreibung (Abstract): | The tonoplast H+-pyrophosphatase (EC 3.6.1.1) from photosynthetically inactive cell cultures of the C3-plant Catharanthus roseus (L.) G. Don., and from leaves of the C3/CAM-intermediate plant Mesembryanthemum crystallinum L. in the C3-state and of the obligate CAM-plant Kalanchoë daigremontiana Hamet et Perrier de la Bâthie, was purified using Mono Q anion-exchange and Superose 6 size-exclusion fast protein liquid chromatography. The combination of both chromatographical techniques led to a single polypeptide with an apparent molecular mass of 72 kDa exhibiting K+-stimulated pyrophosphate hydrolysis activity in preparations from C. roseus and M. crystallinum and to two polypeptides (72 and 70 kDa) in hydrolytic active fractions from K. daigremontiana. These polypeptides cross-reacted with an antiserum against the tonoplast inorganic H+-pyrophosphatase of Vigna radiata L. cv. Wilczek. The possible identity of the 70 kDa polypeptide of K. daigremontiana is discussed. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Botanischer Garten |
| Hinterlegungsdatum: | 19 Nov 2008 15:57 |
| Letzte Änderung: | 11 Aug 2023 14:39 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
 |
Frage zum Eintrag |
Optionen (nur für Redakteure)
 |
Redaktionelle Details anzeigen |
Drucken
Impressum