Sharifian, Hoda ; Lampert, Fabienne ; Stojanovski, Klement ; Regot, Sergi ; Vaga, Stefania ; Buser, Raymond ; Lee, Sung Sik ; Koeppl, Heinz ; Posas, Francesc ; Pelet, Serge ; Peter, Matthias (2015)
Parallel feedback loops control the basal activity of the HOG MAPK signaling cascade.
In: Integrative Biology, 7 (4)
doi: 10.1039/c4ib00299g
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
Tight regulation of the MAP kinase Hog1 is crucial for survival under changing osmotic conditions. Interestingly, we found that Hog1 phosphorylates multiple upstream components, implying feedback regulation within the signaling cascade. Taking advantage of an unexpected link between glucose availability and Hog1 activity, we used quantitative single cell measurements and computational modeling to unravel feedback regulation operating in addition to the well-known adaptation feedback triggered by glycerol accumulation. Indeed, we found that Hog1 phosphorylates its activating kinase Ssk2 on several sites, and cells expressing a non-phosphorylatable Ssk2 mutant are partially defective for feedback regulation and proper control of basal Hog1 activity. Together, our data suggest that Hog1 activity is controlled by intertwined regulatory mechanisms operating with varying kinetics, which together tune the Hog1 response to balance basal Hog1 activity and its steady-state level after adaptation to high osmolarity.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2015 |
| Autor(en): | Sharifian, Hoda ; Lampert, Fabienne ; Stojanovski, Klement ; Regot, Sergi ; Vaga, Stefania ; Buser, Raymond ; Lee, Sung Sik ; Koeppl, Heinz ; Posas, Francesc ; Pelet, Serge ; Peter, Matthias |
| Art des Eintrags: | Bibliographie |
| Titel: | Parallel feedback loops control the basal activity of the HOG MAPK signaling cascade |
| Sprache: | Englisch |
| Publikationsjahr: | April 2015 |
| Ort: | Cambridge |
| Verlag: | Royal Society of Chemistry |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Integrative Biology |
| Jahrgang/Volume einer Zeitschrift: | 7 |
| (Heft-)Nummer: | 4 |
| DOI: | 10.1039/c4ib00299g |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | Tight regulation of the MAP kinase Hog1 is crucial for survival under changing osmotic conditions. Interestingly, we found that Hog1 phosphorylates multiple upstream components, implying feedback regulation within the signaling cascade. Taking advantage of an unexpected link between glucose availability and Hog1 activity, we used quantitative single cell measurements and computational modeling to unravel feedback regulation operating in addition to the well-known adaptation feedback triggered by glycerol accumulation. Indeed, we found that Hog1 phosphorylates its activating kinase Ssk2 on several sites, and cells expressing a non-phosphorylatable Ssk2 mutant are partially defective for feedback regulation and proper control of basal Hog1 activity. Together, our data suggest that Hog1 activity is controlled by intertwined regulatory mechanisms operating with varying kinetics, which together tune the Hog1 response to balance basal Hog1 activity and its steady-state level after adaptation to high osmolarity. |
| Zusätzliche Informationen: | Erstveröffentlichung |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 600 Technik, Medizin, angewandte Wissenschaften > 621.3 Elektrotechnik, Elektronik |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Self-Organizing Systems Lab |
| Hinterlegungsdatum: | 17 Dez 2024 12:42 |
| Letzte Änderung: | 17 Dez 2024 12:42 |
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| Suche nach Titel in: | TUfind oder in Google |
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Parallel feedback loops control the basal activity of the HOG MAPK signaling cascade. (deposited 16 Dez 2024 13:50)
- Parallel feedback loops control the basal activity of the HOG MAPK signaling cascade. (deposited 17 Dez 2024 12:42) [Gegenwärtig angezeigt]
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