Spiegel, Christopher ; Klinge, Lina ; Kluy, Lukas ; Groche, Peter ; Siemers, Carsten ; Coraça-Huber, Débora (2024)
Cell behavior of human-fetal-osteoblasts (hfOB) and Staphylococcus Aureus on nanostructured titanium alloy Ti-13Nb-13Zr (NanoTNZ).
World Titanium Conference 2023. Edinburgh, Scotland (12.06.2023 - 16.06.2023)
doi: 10.7490/f1000research.1119883.1
Konferenzveröffentlichung, Bibliographie
Kurzbeschreibung (Abstract)
Titanium and titanium alloys are the most suitable metallic materials in implant engineering, whereas currently, 1–2 of all prosthetic joint surgeries are followed by an infection. Prosthetic joint infections are mainly caused by Staphylococcus aureus. The formation of biofilms helps bacterial cells to withstand critical environmental conditions. Due to their tolerance against antibacterial substances, biofilms are a significant problem in modern medicine. Nanostructured titanium alloys have shown promising results on controlling bacterial adhesion and growth in comparison to adhesion of osteoblasts. To accomplish the requirements for lighter, more elastic materials with a nanostructured surface, Ti-13Nb-13Zr (TNZ) shows promising properties. In this study we investigated cell adhesion and proliferation of human-fetal-osteoblasts (hFOB 1.19) and Staphylococcus aureus ATCC 29213 on nanostructured Ti-13Nb-13Zr, a second-generation biomedical beta-rich (alpha+beta)-titanium alloy which was produced by equal channel angular swaging (ECAS) followed by recrystallisation and ageing treatments. In addition, CP Titanium Grade 4 and Ti-6Al-4V ELI were investigated as reference materials. To evaluate the adhesion and proliferation of hFOB 1.19, we performed cell counting after 72 h of cultivation with a hemacytometer, following RNA extraction and RT-PCR with suiting primers for cell proliferation and differentiation. In addition, scanning electron microscopy was used to gain insights into cell morphology of hFOB 1.19 on nanostructured Ti-13Nb-13Zr. Bacterial adhesion and proliferation of Staphylococcus aureus ATCC 29213 was studied by colony forming unit counting after 24 h of incubation together with SEM analyses. The results of our investigations will help to optimize the microstructure of Nano-TNZ with respect to good osteoblast adhesion and minimized bacterial colonization and biofilm production to allow the development of TNZ-implants with enhanced biological properties.
| Typ des Eintrags: | Konferenzveröffentlichung |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Spiegel, Christopher ; Klinge, Lina ; Kluy, Lukas ; Groche, Peter ; Siemers, Carsten ; Coraça-Huber, Débora |
| Art des Eintrags: | Bibliographie |
| Titel: | Cell behavior of human-fetal-osteoblasts (hfOB) and Staphylococcus Aureus on nanostructured titanium alloy Ti-13Nb-13Zr (NanoTNZ) |
| Sprache: | Englisch |
| Publikationsjahr: | 2024 |
| Ort: | London |
| Verlag: | F1000 Research Ltd. (Taylor & Francis Group) |
| Buchtitel: | Proceedings of the 15th World Conference on Titanium, Chapter 3: Biomedical and Healthcare Applications |
| Kollation: | 6 Seiten |
| Veranstaltungstitel: | World Titanium Conference 2023 |
| Veranstaltungsort: | Edinburgh, Scotland |
| Veranstaltungsdatum: | 12.06.2023 - 16.06.2023 |
| DOI: | 10.7490/f1000research.1119883.1 |
| Kurzbeschreibung (Abstract): | Titanium and titanium alloys are the most suitable metallic materials in implant engineering, whereas currently, 1–2 of all prosthetic joint surgeries are followed by an infection. Prosthetic joint infections are mainly caused by Staphylococcus aureus. The formation of biofilms helps bacterial cells to withstand critical environmental conditions. Due to their tolerance against antibacterial substances, biofilms are a significant problem in modern medicine. Nanostructured titanium alloys have shown promising results on controlling bacterial adhesion and growth in comparison to adhesion of osteoblasts. To accomplish the requirements for lighter, more elastic materials with a nanostructured surface, Ti-13Nb-13Zr (TNZ) shows promising properties. In this study we investigated cell adhesion and proliferation of human-fetal-osteoblasts (hFOB 1.19) and Staphylococcus aureus ATCC 29213 on nanostructured Ti-13Nb-13Zr, a second-generation biomedical beta-rich (alpha+beta)-titanium alloy which was produced by equal channel angular swaging (ECAS) followed by recrystallisation and ageing treatments. In addition, CP Titanium Grade 4 and Ti-6Al-4V ELI were investigated as reference materials. To evaluate the adhesion and proliferation of hFOB 1.19, we performed cell counting after 72 h of cultivation with a hemacytometer, following RNA extraction and RT-PCR with suiting primers for cell proliferation and differentiation. In addition, scanning electron microscopy was used to gain insights into cell morphology of hFOB 1.19 on nanostructured Ti-13Nb-13Zr. Bacterial adhesion and proliferation of Staphylococcus aureus ATCC 29213 was studied by colony forming unit counting after 24 h of incubation together with SEM analyses. The results of our investigations will help to optimize the microstructure of Nano-TNZ with respect to good osteoblast adhesion and minimized bacterial colonization and biofilm production to allow the development of TNZ-implants with enhanced biological properties. |
| Freie Schlagworte: | nanostructured titanium, Ti-13Nb-13Zr, TNZ, NanoTNZ, medical implants |
| Fachbereich(e)/-gebiet(e): | 16 Fachbereich Maschinenbau 16 Fachbereich Maschinenbau > Institut für Produktionstechnik und Umformmaschinen (PtU) 16 Fachbereich Maschinenbau > Institut für Produktionstechnik und Umformmaschinen (PtU) > Forschungsabteilung Prozessketten und Anlagen |
| Hinterlegungsdatum: | 18 Okt 2024 07:38 |
| Letzte Änderung: | 22 Okt 2024 06:50 |
| PPN: | 522321305 |
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