Vögele, Jennifer ; Ferner, Jan-Peter ; Altincekic, Nadide ; Bains, Jasleen Kaur ; Ceylan, Betül ; Fürtig, Boris ; Grün, J. Tassilo ; Hengesbach, Martin ; Hohmann, Katharina F. ; Hymon, Daniel ; Knezic, Bozana ; Löhr, Frank ; Peter, Stephen A. ; Pyper, Dennis ; Qureshi, Nusrat S. ; Richter, Christian ; Schlundt, Andreas ; Schwalbe, Harald ; Stirnal, Elke ; Sudakov, Alexey ; Wacker, Anna ; Weigand, Julia E. ; Wirmer-Bartoschek, Julia ; Wöhnert, Jens ; Duchardt-Ferner, Elke (2021)
¹H, ¹³C and ¹⁵N and ³¹P chemical shift assignment for stem-loop 4 from the 5′-UTR of SARS-CoV-2.
In: Biomolecular NMR Assignments, 15 (2)
doi: 10.1007/s12104-021-10026-7
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
The SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to fight the disease once it has been contracted will still be required. Promising targets for the development of antiviral agents against SARS-CoV-2 can be found in the viral RNA genome. The 5′- and 3′-genomic ends of the 30 kb SCoV-2 genome are highly conserved among Betacoronaviruses and contain structured RNA elements involved in the translation and replication of the viral genome. The 40 nucleotides (nt) long highly conserved stem-loop 4 (5_SL4) is located within the 5′-untranslated region (5′-UTR) important for viral replication. 5_SL4 features an extended stem structure disrupted by several pyrimidine mismatches and is capped by a pentaloop. Here, we report extensive ¹H, ¹³C and ¹⁵N and ³¹P resonance assignments of 5_SL4 as the basis for in-depth structural and ligand screening studies by solution NMR spectroscopy.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2021 |
| Autor(en): | Vögele, Jennifer ; Ferner, Jan-Peter ; Altincekic, Nadide ; Bains, Jasleen Kaur ; Ceylan, Betül ; Fürtig, Boris ; Grün, J. Tassilo ; Hengesbach, Martin ; Hohmann, Katharina F. ; Hymon, Daniel ; Knezic, Bozana ; Löhr, Frank ; Peter, Stephen A. ; Pyper, Dennis ; Qureshi, Nusrat S. ; Richter, Christian ; Schlundt, Andreas ; Schwalbe, Harald ; Stirnal, Elke ; Sudakov, Alexey ; Wacker, Anna ; Weigand, Julia E. ; Wirmer-Bartoschek, Julia ; Wöhnert, Jens ; Duchardt-Ferner, Elke |
| Art des Eintrags: | Bibliographie |
| Titel: | ¹H, ¹³C and ¹⁵N and ³¹P chemical shift assignment for stem-loop 4 from the 5′-UTR of SARS-CoV-2 |
| Sprache: | Englisch |
| Publikationsjahr: | 2021 |
| Ort: | Dordrecht |
| Verlag: | Springer Netherlands |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biomolecular NMR Assignments |
| Jahrgang/Volume einer Zeitschrift: | 15 |
| (Heft-)Nummer: | 2 |
| DOI: | 10.1007/s12104-021-10026-7 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | The SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to fight the disease once it has been contracted will still be required. Promising targets for the development of antiviral agents against SARS-CoV-2 can be found in the viral RNA genome. The 5′- and 3′-genomic ends of the 30 kb SCoV-2 genome are highly conserved among Betacoronaviruses and contain structured RNA elements involved in the translation and replication of the viral genome. The 40 nucleotides (nt) long highly conserved stem-loop 4 (5_SL4) is located within the 5′-untranslated region (5′-UTR) important for viral replication. 5_SL4 features an extended stem structure disrupted by several pyrimidine mismatches and is capped by a pentaloop. Here, we report extensive ¹H, ¹³C and ¹⁵N and ³¹P resonance assignments of 5_SL4 as the basis for in-depth structural and ligand screening studies by solution NMR spectroscopy. |
| Freie Schlagworte: | SARS-CoV-2, RNA genome, 5′-UTR, 5_SL4, Solution NMR spectroscopy, COVID19-NMR |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 540 Chemie 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > RNA Biochemie |
| Hinterlegungsdatum: | 04 Okt 2024 08:32 |
| Letzte Änderung: | 04 Okt 2024 08:32 |
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| Suche nach Titel in: | TUfind oder in Google |
Verfügbare Versionen dieses Eintrags
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¹H, ¹³C and ¹⁵N and ³¹P chemical shift assignment for stem-loop 4 from the 5′-UTR of SARS-CoV-2. (deposited 30 Sep 2024 12:09)
- ¹H, ¹³C and ¹⁵N and ³¹P chemical shift assignment for stem-loop 4 from the 5′-UTR of SARS-CoV-2. (deposited 04 Okt 2024 08:32) [Gegenwärtig angezeigt]
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