Pavez Lorie, Elizabeth ; Stricker, Nicola ; Plitta-Michalak, Beata ; Chen, I.-Peng ; Volkmer, Beate ; Greinert, Rüdiger ; Jauch, Anna ; Boukamp, Petra ; Rapp, Alexander (2024)
Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw.
In: Scientific Reports, 2020, 10 (1)
doi: 10.26083/tuprints-00024029
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
We here present the spontaneously immortalised cell line, HaSKpw, as a novel model for the multistep process of skin carcinogenesis. HaSKpw cells were established from the epidermis of normal human adult skin that, without crisis, are now growing unrestricted and feeder-independent. At passage 22, clonal populations were established and clone7 (HaSKpwC7) was further compared to the also spontaneously immortalized HaCaT cells. As important differences, the HaSKpw cells express wild-type p53, remain pseudodiploid, and show a unique chromosomal profile with numerous complex aberrations involving chromosome 20. In addition, HaSKpw cells overexpress a pattern of genes and miRNAs such as KRT34, LOX, S100A9, miR21, and miR155; all pointing to a tumorigenic status. In concordance, HaSKpw cells exhibit reduced desmosomal contacts that provide them with increased motility and a highly migratory/invasive phenotype as demonstrated in scratch- and Boyden chamber assays. In 3D organotypic cultures, both HaCaT and HaSKpw cells form disorganized epithelia but only the HaSKpw cells show tumorcell-like invasive growth. Together, HaSKpwC7 and HaCaT cells represent two spontaneous (non-genetically engineered) “premalignant” keratinocyte lines from adult human skin that display different stages of the multistep process of skin carcinogenesis and thus represent unique models for analysing skin cancer development and progression.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Pavez Lorie, Elizabeth ; Stricker, Nicola ; Plitta-Michalak, Beata ; Chen, I.-Peng ; Volkmer, Beate ; Greinert, Rüdiger ; Jauch, Anna ; Boukamp, Petra ; Rapp, Alexander |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw |
| Sprache: | Englisch |
| Publikationsjahr: | 25 September 2024 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 16 September 2020 |
| Ort der Erstveröffentlichung: | London |
| Verlag: | Springer Nature |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Scientific Reports |
| Jahrgang/Volume einer Zeitschrift: | 10 |
| (Heft-)Nummer: | 1 |
| Kollation: | 20 Seiten |
| DOI: | 10.26083/tuprints-00024029 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/24029 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung DeepGreen |
| Kurzbeschreibung (Abstract): | We here present the spontaneously immortalised cell line, HaSKpw, as a novel model for the multistep process of skin carcinogenesis. HaSKpw cells were established from the epidermis of normal human adult skin that, without crisis, are now growing unrestricted and feeder-independent. At passage 22, clonal populations were established and clone7 (HaSKpwC7) was further compared to the also spontaneously immortalized HaCaT cells. As important differences, the HaSKpw cells express wild-type p53, remain pseudodiploid, and show a unique chromosomal profile with numerous complex aberrations involving chromosome 20. In addition, HaSKpw cells overexpress a pattern of genes and miRNAs such as KRT34, LOX, S100A9, miR21, and miR155; all pointing to a tumorigenic status. In concordance, HaSKpw cells exhibit reduced desmosomal contacts that provide them with increased motility and a highly migratory/invasive phenotype as demonstrated in scratch- and Boyden chamber assays. In 3D organotypic cultures, both HaCaT and HaSKpw cells form disorganized epithelia but only the HaSKpw cells show tumorcell-like invasive growth. Together, HaSKpwC7 and HaCaT cells represent two spontaneous (non-genetically engineered) “premalignant” keratinocyte lines from adult human skin that display different stages of the multistep process of skin carcinogenesis and thus represent unique models for analysing skin cancer development and progression. |
| Freie Schlagworte: | Cell biology, Skin cancer, Squamous cell carcinoma |
| ID-Nummer: | Artikel-ID: 15196 |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-240293 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin, Gesundheit |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Cell Biology and Epigenetics |
| Hinterlegungsdatum: | 25 Sep 2024 11:39 |
| Letzte Änderung: | 26 Sep 2024 07:33 |
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| Suche nach Titel in: | TUfind oder in Google |
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- Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw. (deposited 25 Sep 2024 11:39) [Gegenwärtig angezeigt]
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