Szabo, Gréta V. ; Burg, Thomas P. (2024)
Time Resolved Cryo‐Correlative Light and Electron Microscopy.
In: Advanced Functional Materials, (30)
doi: 10.1002/adfm.202313705
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Complex materials exhibit fascinating features especially in situations far from equilibrium. Thus, methods for investigating structural dynamics with sub-second time resolution are becoming a question of interest at varying spatial scales. With novel microscopy techniques steadily improving, the temporal and spatial limits of multiple imaging methods are investigated with an emphasis on the important role of correlative imaging and cryo-fixation. A deep-dive is taken into cryo-correlative light and electron microscopy (CLEM) as a starting point for multimodal investigations of ultrastructural dynamics at high spatiotemporal resolution. The focus is on highlighting the different microscopy methods that capture the following key aspects: 1) samples are as close to native state as possible 2) dynamic process information is captured, 3) high structural resolution is enabled. Additionally, the size of samples that can be imaged under these conditions is looked at and approaches not only focusing on single molecules, but larger structures are highlighted.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Szabo, Gréta V. ; Burg, Thomas P. |
| Art des Eintrags: | Bibliographie |
| Titel: | Time Resolved Cryo‐Correlative Light and Electron Microscopy |
| Sprache: | Englisch |
| Publikationsjahr: | 2024 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Advanced Functional Materials |
| (Heft-)Nummer: | 30 |
| Band einer Reihe: | 34 |
| DOI: | 10.1002/adfm.202313705 |
| URL / URN: | https://doi.org/10.1002/adfm.202313705 |
| Kurzbeschreibung (Abstract): | Complex materials exhibit fascinating features especially in situations far from equilibrium. Thus, methods for investigating structural dynamics with sub-second time resolution are becoming a question of interest at varying spatial scales. With novel microscopy techniques steadily improving, the temporal and spatial limits of multiple imaging methods are investigated with an emphasis on the important role of correlative imaging and cryo-fixation. A deep-dive is taken into cryo-correlative light and electron microscopy (CLEM) as a starting point for multimodal investigations of ultrastructural dynamics at high spatiotemporal resolution. The focus is on highlighting the different microscopy methods that capture the following key aspects: 1) samples are as close to native state as possible 2) dynamic process information is captured, 3) high structural resolution is enabled. Additionally, the size of samples that can be imaged under these conditions is looked at and approaches not only focusing on single molecules, but larger structures are highlighted. |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Integrierte Mikro-Nano-Systeme |
| Hinterlegungsdatum: | 11 Sep 2024 08:22 |
| Letzte Änderung: | 23 Okt 2024 13:07 |
| PPN: | 522440746 |
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