Hassan, Mohamed Atef ; Kern, Michal ; Chu, Anh ; Kalra, Gatik ; Shabratova, Ekaterina ; Tsarapkin, Aleksei ; MacKinnon, Neil ; Lips, Klaus ; Teutloff, Christian ; Bittl, Robert ; Korvink, Jan Gerrit ; Anders, Jens (2022)
Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors.
In: Frequenz, 76 (11-12)
doi: 10.1515/freq-2022-0096
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
Electron paramagnetic resonance (EPR) is the gold standard for studying paramagnetic species. As an example, in structural biology, it allows to extract information about distance distributions on the nanometer scale via site-directed spin labeling. Conventional pulsed EPR of biological samples is currently limited to relatively large sample concentrations and cryogenic temperatures, mainly due to low sensitivity and the significant dead time associated with conventional resonator-based EPR setups, essentially precluding in-cell EPR under physiological conditions. This paper presents our latest progress toward single-cell pulsed EPR using VCO-based EPR-on-a-chip (EPRoC) sensors. Together with an analytical model for VCO-based pulsed EPR, we present an experimental scheme to perform dead time- free pulsed EPR measurements using EPRoC detectors. The proposed scheme is validated using extensive numerical simulations and proof-of-concept experiments on the spin dynamics of an organic radical at room temperature using a custom-designed EPRoC detector operating in the Ka-band around 30.4 GHz. Additionally, we discuss methods to improve the excitation field homogeneity and sample handling through chip post-processing and custom-designed microfluidics. Finally, we present our progress towards compact, portable pulsed EPR spectrometers incorporating EPRoC detectors, microfluidics, and custom-designed permanent magnets. Such portable EPR spectrometers can pave the way toward new EPR applications, including point-of care diagnostics.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2022 |
| Autor(en): | Hassan, Mohamed Atef ; Kern, Michal ; Chu, Anh ; Kalra, Gatik ; Shabratova, Ekaterina ; Tsarapkin, Aleksei ; MacKinnon, Neil ; Lips, Klaus ; Teutloff, Christian ; Bittl, Robert ; Korvink, Jan Gerrit ; Anders, Jens |
| Art des Eintrags: | Bibliographie |
| Titel: | Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors |
| Sprache: | Englisch |
| Publikationsjahr: | 2022 |
| Ort: | Darmstadt |
| Verlag: | De Gruyter |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Frequenz |
| Jahrgang/Volume einer Zeitschrift: | 76 |
| (Heft-)Nummer: | 11-12 |
| DOI: | 10.1515/freq-2022-0096 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | Electron paramagnetic resonance (EPR) is the gold standard for studying paramagnetic species. As an example, in structural biology, it allows to extract information about distance distributions on the nanometer scale via site-directed spin labeling. Conventional pulsed EPR of biological samples is currently limited to relatively large sample concentrations and cryogenic temperatures, mainly due to low sensitivity and the significant dead time associated with conventional resonator-based EPR setups, essentially precluding in-cell EPR under physiological conditions. This paper presents our latest progress toward single-cell pulsed EPR using VCO-based EPR-on-a-chip (EPRoC) sensors. Together with an analytical model for VCO-based pulsed EPR, we present an experimental scheme to perform dead time- free pulsed EPR measurements using EPRoC detectors. The proposed scheme is validated using extensive numerical simulations and proof-of-concept experiments on the spin dynamics of an organic radical at room temperature using a custom-designed EPRoC detector operating in the Ka-band around 30.4 GHz. Additionally, we discuss methods to improve the excitation field homogeneity and sample handling through chip post-processing and custom-designed microfluidics. Finally, we present our progress towards compact, portable pulsed EPR spectrometers incorporating EPRoC detectors, microfluidics, and custom-designed permanent magnets. Such portable EPR spectrometers can pave the way toward new EPR applications, including point-of care diagnostics. |
| Freie Schlagworte: | dead time free EPR, EPR, EPR-on-a-chip, EPRoC, Rabi oscillations, VCO-based pulsed EPR |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 530 Physik 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften und Maschinenbau |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Institut für Mikrowellentechnik und Photonik (IMP) |
| Hinterlegungsdatum: | 02 Aug 2024 12:50 |
| Letzte Änderung: | 02 Aug 2024 12:50 |
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| Suche nach Titel in: | TUfind oder in Google |
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Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors. (deposited 01 Mär 2023 10:02)
- Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors. (deposited 02 Aug 2024 12:50) [Gegenwärtig angezeigt]
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