Oloketuyi, Sandra ; Bernedo, Robert ; Christmann, Andreas ; Borkowska, Justyna ; Cazzaniga, Giulia ; Schuchmann, Horst Wilhelm ; Niedziółka-Jönsson, Joanna ; Szot-Karpińska, Katarzyna ; Kolmar, Harald ; Marco, Ario de (2022)
Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection.
In: Biosensors, 11 (12)
doi: 10.3390/bios11120496
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production, simple engineering, and the possibility to obtain higher binder density on capture surfaces. Starting from the same pre-immune library, we compared the selection output resulting from two independent panning strategies, one exclusively exploiting the phage display and another in which a first round of phage display was followed by a second round of yeast display. There was a partial output convergence between the two methods, since two clones were identified using both panning protocols but the first provided several further different sequences, whereas the second favored the recovery of many copies of few clones. The isolated anti-CRP nanobodies had affinity in the low nanomolar range and were suitable for ELISA and immunoprecipitation. One of them was fused to SpyTag and exploited in combination with SpyCatcher as the immunocapture element to quantify CRP using electrochemical impedance spectroscopy. The sensitivity of the biosensor was calculated as low as 0.21 μg/mL.

Typ des Eintrags:	Artikel
Erschienen:	2022
Autor(en):	Oloketuyi, Sandra ; Bernedo, Robert ; Christmann, Andreas ; Borkowska, Justyna ; Cazzaniga, Giulia ; Schuchmann, Horst Wilhelm ; Niedziółka-Jönsson, Joanna ; Szot-Karpińska, Katarzyna ; Kolmar, Harald ; Marco, Ario de
Art des Eintrags:	Bibliographie
Titel:	Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection
Sprache:	Englisch
Publikationsjahr:	2022
Verlag:	MDPI
Titel der Zeitschrift, Zeitung oder Schriftenreihe:	Biosensors
Jahrgang/Volume einer Zeitschrift:	11
(Heft-)Nummer:	12
Kollation:	14 Seiten
DOI:	10.3390/bios11120496
Zugehörige Links:	TUprints Zweitveröffentlichung
Kurzbeschreibung (Abstract):	C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production, simple engineering, and the possibility to obtain higher binder density on capture surfaces. Starting from the same pre-immune library, we compared the selection output resulting from two independent panning strategies, one exclusively exploiting the phage display and another in which a first round of phage display was followed by a second round of yeast display. There was a partial output convergence between the two methods, since two clones were identified using both panning protocols but the first provided several further different sequences, whereas the second favored the recovery of many copies of few clones. The isolated anti-CRP nanobodies had affinity in the low nanomolar range and were suitable for ELISA and immunoprecipitation. One of them was fused to SpyTag and exploited in combination with SpyCatcher as the immunocapture element to quantify CRP using electrochemical impedance spectroscopy. The sensitivity of the biosensor was calculated as low as 0.21 μg/mL.
Freie Schlagworte:	phage display, yeast display, nanobodies, biopanning, CRP
Sachgruppe der Dewey Dezimalklassifikatin (DDC):	500 Naturwissenschaften und Mathematik > 540 Chemie 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
Fachbereich(e)/-gebiet(e):	07 Fachbereich Chemie 07 Fachbereich Chemie > Clemens-Schöpf-Institut > Fachgebiet Biochemie 07 Fachbereich Chemie > Clemens-Schöpf-Institut
Hinterlegungsdatum:	02 Aug 2024 12:40
Letzte Änderung:	02 Aug 2024 12:40
PPN:
Export:	T2T_XMLIBW_RDAEndNoteEP3 XMLAtomHTML CitationBibTeXMODSASCII CitationRDF+XMLDublin CoreMultiline CSVJSONSimple Metadata
Suche nach Titel in:	TUfind oder in Google

Verfügbare Versionen dieses Eintrags

• Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection. (deposited 29 Apr 2022 09:08)
  • Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection. (deposited 02 Aug 2024 12:40) [Gegenwärtig angezeigt]

Frage zum Eintrag

Optionen (nur für Redakteure)

Redaktionelle Details anzeigen