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Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches

Karakaya, Emine ; Bider, Faina ; Frank, Andreas ; Teßmar, Jörg ; Schöbel, Lisa ; Forster, Leonard ; Schrüfer, Stefan ; Schmidt, Hans-Werner ; Schubert, Dirk Wolfram ; Blaeser, Andreas ; Boccaccini, Aldo R. ; Detsch, Rainer (2022)
Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches.
In: Gels, 8 (4)
doi: 10.3390/gels8040206
Artikel, Bibliographie

Dies ist die neueste Version dieses Eintrags.

Kurzbeschreibung (Abstract)

A novel approach, in the context of bioprinting, is the targeted printing of a defined number of cells at desired positions in predefined locations, which thereby opens up new perspectives for life science engineering. One major challenge in this application is to realize the targeted printing of cells onto a gel substrate with high cell survival rates in advanced bioinks. For this purpose, different alginate-dialdehyde—polyethylene glycol (ADA-PEG) inks with different PEG modifications and chain lengths (1–8 kDa) were characterized to evaluate their application as bioinks for drop on demand (DoD) printing. The biochemical properties of the inks, printing process, NIH/3T3 fibroblast cell distribution within a droplet and shear forces during printing were analyzed. Finally, different hydrogels were evaluated as a printing substrate. By analysing different PEG chain lengths with covalently crosslinked and non-crosslinked ADA-PEG inks, it was shown that the influence of Schiff’s bases on the viscosity of the corresponding materials is very low. Furthermore, it was shown that longer polymer chains resulted in less stable hydrogels, leading to fast degradation rates. Several bioinks highly exhibit biocompatibility, while the calculated nozzle shear stress increased from approx. 1.3 and 2.3 kPa. Moreover, we determined the number of cells for printed droplets depending on the initial cell concentration, which is crucially needed for targeted cell printing approaches.

Typ des Eintrags: Artikel
Erschienen: 2022
Autor(en): Karakaya, Emine ; Bider, Faina ; Frank, Andreas ; Teßmar, Jörg ; Schöbel, Lisa ; Forster, Leonard ; Schrüfer, Stefan ; Schmidt, Hans-Werner ; Schubert, Dirk Wolfram ; Blaeser, Andreas ; Boccaccini, Aldo R. ; Detsch, Rainer
Art des Eintrags: Bibliographie
Titel: Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches
Sprache: Englisch
Publikationsjahr: 2022
Verlag: MDPI
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Gels
Jahrgang/Volume einer Zeitschrift: 8
(Heft-)Nummer: 4
Kollation: 24 Seiten
DOI: 10.3390/gels8040206
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Kurzbeschreibung (Abstract):

A novel approach, in the context of bioprinting, is the targeted printing of a defined number of cells at desired positions in predefined locations, which thereby opens up new perspectives for life science engineering. One major challenge in this application is to realize the targeted printing of cells onto a gel substrate with high cell survival rates in advanced bioinks. For this purpose, different alginate-dialdehyde—polyethylene glycol (ADA-PEG) inks with different PEG modifications and chain lengths (1–8 kDa) were characterized to evaluate their application as bioinks for drop on demand (DoD) printing. The biochemical properties of the inks, printing process, NIH/3T3 fibroblast cell distribution within a droplet and shear forces during printing were analyzed. Finally, different hydrogels were evaluated as a printing substrate. By analysing different PEG chain lengths with covalently crosslinked and non-crosslinked ADA-PEG inks, it was shown that the influence of Schiff’s bases on the viscosity of the corresponding materials is very low. Furthermore, it was shown that longer polymer chains resulted in less stable hydrogels, leading to fast degradation rates. Several bioinks highly exhibit biocompatibility, while the calculated nozzle shear stress increased from approx. 1.3 and 2.3 kPa. Moreover, we determined the number of cells for printed droplets depending on the initial cell concentration, which is crucially needed for targeted cell printing approaches.

Freie Schlagworte: bioprinting, drop on demand, sodium alginate, polyethylene glycol, shear stress
Sachgruppe der Dewey Dezimalklassifikatin (DDC): 500 Naturwissenschaften und Mathematik > 540 Chemie
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften und Maschinenbau
Fachbereich(e)/-gebiet(e): 16 Fachbereich Maschinenbau
16 Fachbereich Maschinenbau > Institut für Druckmaschinen und Druckverfahren (IDD)
16 Fachbereich Maschinenbau > Institut für Druckmaschinen und Druckverfahren (IDD) > Biomedizinische Drucktechnologie (BMT)
Interdisziplinäre Forschungsprojekte
Interdisziplinäre Forschungsprojekte > Centre for Synthetic Biology
Hinterlegungsdatum: 02 Aug 2024 12:39
Letzte Änderung: 02 Aug 2024 12:39
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